Application

Affinity gel is suitable for the purification of histidine tagged proteins in native and denaturing conditions.

Nickel Affinity Gel for the purication of histidine tagged proteins. HIS-Select? High Flow (HF) brings the superior selectivity of HIS-Select technology to a highly cross-linked agarose for higher flow rates and mechanical stability under pressure. HIS-Select HF is designed for production scale purification and FPLC^? applications. As with other HIS-Select products, the non-charged, hydrophilic linkage of the nickel-nitrilotriacetic acid (Ni-NTA) chelate group to the agarose ensures true one-step purification.

Features and Benefits

? High selectivity for higher purity
? Unique non-charged hydrophilic linkage reduced non-specific binding
? Binding Capacity for histidine-tagged protein is greater than 15 mg/ml
? Highly cross-linked agarose matrix allows for purification under higher pressures
? Binding under denaturing or non-denaturing conditions
? One-step purification

General description

HIS-Select High Flow Nickel Affinity Gel is an immobilized metal-ion affinity chromatography (IMAC) gel that is designed to specifically bind histidine containing proteins in chromatographic systems with pressures up to 200 psi and a maximum linear flow rate of 3,000 cm/hr.

Preparation Note

Ethanol must be removed prior to use. Wash affinity gel in deionized water to remove the ethanol, then equilibrate with equilibration buffer that is provided.

Physical form

30% 乙醇中的 50% 悬浮液

法律信息

FPLC is a trademark of GE Healthcare

HIS-Select is a registered trademark of Sigma-Aldrich Co. LLC