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Experiments conducted using Duolink In Situ reagents can detect and visualize protein interactions, protein expression levels and post translational modifications at the single molecule level in fixed cells and tissue samples.
To perform a complete Duolink In Situ experiment you will need two primary antibodies (IHC or ICC/IF validated) that recognize two target epitopes. Additional reagents required include a pair of PLA® probes, one PLUS and one MINUS , your choice of Detection Reagents. Optional reagents include Wash Buffers and Mounting Medium.
Duolink In Situ Microplate Nuclear Stain and Anti-Fade are intended to be used after staining cells with Duolink In Situ in microtiter plates.
Analysis is carried out using a high resolution laser-scanning microscope. Quick and reliable quantification can be performed using the Duolink ImageTool.
Application Note
Follow these guidelines
Find answers to commonly asked question on our Duolink FAQ page
Duolink is a registered trademark of Sigma-Aldrich Co. LLC
PLA is a registered trademark of Sigma-Aldrich Co. LLC
fluorescence | λex 360 nm; λem 460 nm |
suitability | suitable for fluorescence-detection automated sequencing |
suitable for microtiter plates | |
shipped in | dry ice |
storage temp. | ?20°C |
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