Application

Experiments conducted using Duolink In Situ reagents can detect and visualize protein interactions, protein expression levels and post translational modifications at the single molecule level in fixed cells and tissue samples.

To perform a complete Duolink In Situ experiment you will need two primary antibodies (IHC or ICC/IF validated) that recognize two target epitopes. Additional reagents required include a pair of PLA^® probes, one PLUS and one MINUS , your choice of Detection Reagents. Optional reagents include Wash Buffers and Mounting Medium.

Analysis is carried out using standard immunofluorescence assay equipment. Quick and reliable quantification can be performed using the Duolink Image Tool.

Duolink In Situ Wash Buffers for Fluorescence are suitable to be used with any of the Detection Reagents for fluorescence application.


Application Note
Dissolve each of the pouches in 1 L of high purity water. Keep dissolved wash buffer refrigerated but bring to room temperature before using them in the Duolink In Situ assay.

Find answers to commonly asked question on our Duolink FAQ page

法律信息

Duolink is a registered trademark of Sigma-Aldrich Co. LLC

PLA is a registered trademark of Sigma-Aldrich Co. LLC