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Protein kinase C delta (PKC delta) is a member of the protein kinase C (PKC) family of serine-threonine kinases. It is a 79 kd protein kinase that shows strict dependence on the presence of phospholipids, but shows no activation by Ca2+. Phosphatidylinositol is the most potent activator of PKC delta. Northern blot analysis indicated that PKC delta is widely distributed in almost all the tissues and is a major isoform of PKC expressed in hemopoietic cells. PKC delta is involved in fundamental cellular functions regulated by diacylglycerols and mimicked by phorbol esters.
After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.
Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC
recombinant | expressed in baculovirus infected Sf9 cells |
grade | PRECISIO® |
assay | ≥70% (SDS-PAGE) |
form | buffered aqueous glycerol solution |
activity | 213-388 nmol/min·mg |
mol wt | mol wt ~104 kDa |
NCBI accession no. | NM_006254 |
shipped in | dry ice |
storage temp. | ?70°C |
Gene Information | human ... PRKCD(5580) |
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