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PKC alpha is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC-alpha has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. PKC alpha has been assigned to the chromosome region 17q22-q23.2 and has been identified as a fundamental regulator of cardiac contractility and Ca2+ handling in myocytes.
After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.
Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC
recombinant | expressed in baculovirus infected Sf9 cells |
grade | PRECISIO® |
assay | ≥70% (SDS-PAGE) |
form | buffered aqueous glycerol solution |
activity | 186-290 nmol/min·mg |
mol wt | mol wt ~103 kDa |
NCBI accession no. | BC078065 |
shipped in | dry ice |
storage temp. | ?70°C |
Gene Information | Xenopus laevis ... prkca(447031) |
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