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Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.
Protein kinase A (PKA) is a mediator of cAMP (cyclic adenosine monophosphate) and contains two regulatory and two catalytic subunits. Mutations in PRKACA (protein kinase cAMP-activated catalytic subunit α) are associated with Cushing′s syndrome. PRKACA encodes two splice variants, Cα1 and Cα2. Cα1 is widely expressed and Cα2 is restricted to the sperm cells.
PRECISIO 为以下机构的注册商标: Sigma-Aldrich Co. LLC
recombinant | expressed in baculovirus infected Sf9 cells |
grade | PRECISIO® Kinase |
assay | ≥70% (SDS-PAGE) |
form | buffered aqueous glycerol solution |
activity | 1626-2201 nmol/min·mg |
mol wt | mol wt ~69 kDa |
shipped in | dry ice |
storage temp. | ?70°C |
Gene Information | human ... PRKACA(5566) |
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