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NEK1 or NIMA (never in mitosis gene a)-related kinase 1 is a serine/threonine kinase involved in cell cycle regulation and is found in a centrosomal complex with FEZ1, a neuronal protein that plays a role in axonal development. NEK1 is involved early in the DNA damage response pathway. NEK1 cycles through the nucleus via its nuclear localization and export signals. NEK1 protein participates in different signaling pathways to regulate diverse cellular processes and plays an important role in the kidney where it has opened a new avenue for studying cystogenesis and identifying possible modes of therapy.
After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.
Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC
recombinant | expressed in baculovirus infected Sf9 cells |
grade | PRECISIO® |
assay | ≥70% (SDS-PAGE) |
form | buffered aqueous glycerol solution |
activity | 56-84 nmol/min·mg |
mol wt | mol wt ~85 kDa |
NCBI accession no. | NM_175089 |
shipped in | dry ice |
storage temp. | ?70°C |
Gene Information | mouse ... Nek1(18004) |
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