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AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis. AMPK exists as a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits. Binding of AMP to the γ subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) and via signaling pathways in response to adiponectin, leptin and CAMKKβ.
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
Supplied in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
PRECISIO 为以下机构的注册商标: Sigma-Aldrich Co. LLC
recombinant | expressed in baculovirus infected Sf9 cells |
grade | PRECISIO® Kinase |
assay | ≥70% (SDS-PAGE) |
form | buffered aqueous glycerol solution |
activity | 255-345 nmol/min·mg |
mol wt | mol wt ~36 kDa (B2) |
mol wt ~65 kDa (G2) | |
mol wt ~69 kDa (A2) | |
NCBI accession no. | NM_001040633 |
NM_005399 | |
NM_006252 | |
shipped in | dry ice |
storage temp. | ?70°C |
Gene Information | human ... PRKAA2(5563), PRKAB2(5565), PRKAG2(51422) |
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