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The antibody for MCM4 (PLA0039) is used at dilution 1:10,000 and the antibody for CDK2 (PLA0213) is used at dilution 1:1,000 for PLA applications.
Product PLAK0860, Powered by Bethyl Laboratories, has been pre-qualified for use in proximity ligation assay (PLA®). PLA® technology enables visualization of protein interactions in fixed cells and tissue samples while under endogenous protein expression levels.
The secondary-conjugate (indirect) method of PLA® requires the use of a pair of Duolink® probes, one PLUS and one MINUS, and your choice of Detection Reagents. Accessories include Wash Buffers and Mounting Media.
This kit contains antibody PLA0039 and antibody PLA0213, which have been pre-qualified as an antibody pair for use in Proximity Ligation Assay. To view all Duolink products, visit sigma-aldrich.com/duolink.
Duolink is a registered trademark of Sigma-Aldrich Co. LLC
PLA is a registered trademark of Sigma-Aldrich Co. LLC
| grade | Powered by Bethyl Laboratories, Inc. |
| application(s) | proximity ligation assay: suitable |
| shipped in | wet ice |
| storage temp. | 2-8°C |
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