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Protect from light.
SYPRO Ruby protein gel stain is a ready-to-use, ultrasensitive, luminescent stain for the detection of proteins separated by polyacrylamide gel electrophoresis (PAGE). This stain, designed especially for use in 2-D PAGE, has proven to be an excellent choice for 1-D PAGE and isoelectric focusing (IEF) gels as well. SYPRO Ruby protein gel stain attains sensitivity comparable to many silver stain techniques. However, unlike silver staining, the SYPRO Ruby gel stain:
? uses a simple staining protocol, with no possibility of overstaining
? delivers a linear quantitation range of over three orders of magnitude
? shows less protein-to-protein variability
? stains glycoproteins, lipoproteins, calcium-binding proteins, fibrillar proteins, and other difficult-to-stain proteins
? will not stain extraneous nucleic acids
? does not interfere with subsequent analysis of proteins by Edman-based sequencing or mass spectrometry
SYPRO is a registered trademark of Life Technologies
shelf life | ≥6 mo. (when stored at room temperature and protected from light) |
fluorescence | λex 280,450 nm; λem 610 nm |
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