EINECS 编号 200-664-3
MDL number MFCD00284716
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U.S. Patent No. 5,436,134. Licensed from Molecular Probes, Inc.
SYBR is a registered trademark of Life Technologies
On denaturing agarose/formaldehyde gels and polyacrylamide/urea gels, the sensitivity of SYBR Green II RNA gel stain is reduced, though still superior to that of ethidium bromide. Without any washing or destaining steps, SYBR Green II can detect as little as 1 ng RNA per band in agarose/formaldehyde gel or polyacrylamide/urea gel using 254 nm epi-illumination, and about 4 ng RNA per band using 300 nm trans-illumination. Staining agarose/ formaldehyde gels with SYBR Green II does not interfere with the transfer of RNA to membranes or subsequent hybridization in Northern blot analysis as long as 0.1%-0.3% SDS is included in prehybridization and hybridization buffers to remove the dye.
Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has inherently safer chemistry, compared to the standard use of ethidium bromide for staining. For more information see publication found in related content.
usage | 1 mL sufficient for 100 mini-gels |
greener alternative product characteristics | Designing Safer Chemicals Learn more about the Principles of Green Chemistry. |
concentration | 10,000 × in DMSO |
storage temp. | ?20°C |
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