Application

For SYBR based capillary qPCR, optimized for use on Roche Lightcycler

Features and Benefits

? Convenient 2× concentrate ReadyMix specifically designed for use with capillary instruments such as the Roche LightCycler and is ideal for high throughput applications
? Increased specificity & target yield - JumpStart Taq polymerase prevents non-specific product resulting in more accurate C_T values and improved quantitation

Other Notes

SYBR Green JumpStart Taq ReadyMix, Capillary formulation combines the advantages of a hot start enzyme, JumpStart Taq, in a 2× concentrate ReadyMix specifically designed for use with capillary instruments, such as the Roche LightCycler^® real-time thermal cycler. SYBR Green JumpStart Taq ReadyMix is an optimized formulation containing SYBR Green I dye, JumpStart Taq DNA Polymerase, 99% pure deoxynucleotides, buffer and stabilizers.

SYBR Green Taq ReadyMix is recommended for single product real-time amplification experiments and may also be used for evaluation of primer sequences prior to manufacture of fluorescent-labeled probes. Fluorescent labeled probes are not recommended for use with SYBR Green I dye.

包装

A tube of 25 mM MgCl₂ is provided for easy optimization of the QPCR reaction.

Default reaction volume is 20 μL

100RXN is packaged as 1 X 1 mL
400RXN is packaged as 1 X 4 mL

Principle

SYBR Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. SYBR Green I has an excitation and emission maxima of 494 nm and 521 nm, respectively. Specificity of Sigma′s SYBR based QPCR detection is greatly enhanced by the incorporation of a hot-start mediated taq polymerase, JumpStart Taq.

The JumpStart Taq antibody inactivates the DNA polymerase at room temperature. When the temperature is raised above 70 °C in the first denaturation step of the cycling process, the complex dissociates and the polymerase becomes fully active. JumpStart Taq DNA polymerase prevents non-specific amplification resulting in more accurate C_T values.

To prepare a reaction, 10 μL of ReadyMix is added to primers, template and water for a final reaction volume of 20 μL.

Legal Information

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,994,056 and 6,171,785.. 购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的，仅此等数量的产品用于购买者内部研究用途的的免责许可。我们未明确表示、暗示或以禁止反言的形式授予您其他专利声明下的任何权利（如美国专利号6,814,934号下的设备和系统专利声明）、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。

法律信息

JumpStart is a trademark of Sigma-Aldrich Co. LLC

LightCycler is a registered trademark of Roche

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

SYBR is a registered trademark of Life Technologies

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.