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For SYBR based capillary qPCR, optimized for use on Roche Lightcycler
? Convenient 2× concentrate ReadyMix specifically designed for use with capillary instruments such as the Roche LightCycler and is ideal for high throughput applications
? Increased specificity & target yield - JumpStart Taq polymerase prevents non-specific product resulting in more accurate CT values and improved quantitation
SYBR Green JumpStart Taq ReadyMix, Capillary formulation combines the advantages of a hot start enzyme, JumpStart Taq, in a 2× concentrate ReadyMix specifically designed for use with capillary instruments, such as the Roche LightCycler® real-time thermal cycler. SYBR Green JumpStart Taq ReadyMix is an optimized formulation containing SYBR Green I dye, JumpStart Taq DNA Polymerase, 99% pure deoxynucleotides, buffer and stabilizers.
SYBR Green Taq ReadyMix is recommended for single product real-time amplification experiments and may also be used for evaluation of primer sequences prior to manufacture of fluorescent-labeled probes. Fluorescent labeled probes are not recommended for use with SYBR Green I dye.
A tube of 25 mM MgCl2 is provided for easy optimization of the QPCR reaction.
Default reaction volume is 20 μL
100RXN is packaged as 1 X 1 mL
400RXN is packaged as 1 X 4 mL
SYBR Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. SYBR Green I has an excitation and emission maxima of 494 nm and 521 nm, respectively. Specificity of Sigma′s SYBR based QPCR detection is greatly enhanced by the incorporation of a hot-start mediated taq polymerase, JumpStart Taq.
The JumpStart Taq antibody inactivates the DNA polymerase at room temperature. When the temperature is raised above 70 °C in the first denaturation step of the cycling process, the complex dissociates and the polymerase becomes fully active. JumpStart Taq DNA polymerase prevents non-specific amplification resulting in more accurate CT values.
To prepare a reaction, 10 μL of ReadyMix is added to primers, template and water for a final reaction volume of 20 μL.
本产品的使用受到如下一项或多项美国专利及其对应的境
JumpStart is a trademark of Sigma-Aldrich Co. LLC
LightCycler is a registered trademark of Roche
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.
form | liquid |
feature | hotstart |
concentration | 1 units/reaction (20 μL reaction volume) |
color | colorless |
compatibility | for use with Roche LightCycler 480 |
shipped in | wet ice |
storage temp. | ?20°C |
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