Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Components

One mg will inhibit minimum 1.0 mg of trypsin with activity of approx.10,000 BAEE units per mg protein.

Analysis Note

One mg of trypsin inhibitor will inhibit a minimum of 1.0 mg Trypsin of activity ~10,000 BAEE units/mg trypsin.

包装

1, 5, 10 g in poly bottle

500 mg in poly bottle

Preparation Note

胰蛋白酶抑制剂可以 10mg/mL 或更高的浓度溶于水和磷酸盐缓冲液。浓度较高时溶液可能变浑浊，呈黄色至琥珀色。

Unit Definition

One trypsin unit = A₂₅₃ of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C.

One trypsin unit will produce a ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.

Biochem/physiol Actions

大豆胰蛋白酶抑制剂可抑制胰蛋白酶活性，而很少抑制胰凝乳蛋白酶和纤溶酶活性。它可以 1:1 的化学计量与胰蛋白酶形成复合物。形成这种复合物后，胰蛋白酶可裂解抑制剂中的精氨酸-异亮氨酸单键。这种复合物的解离可生成改性或天然的抑制剂。在胰蛋白酶结合的最佳 pH 值 (pH 8.0) 下，缔合常数 ≥ 10×10⁸。