Analysis Note

One mg will inhibit ≥1.0 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.

Other Notes

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包装

5, 10, 25 mg in glass bottle

Preparation Note

Further purification of T9128 yielding an electrophoretically pure Kunitz inhibitor with increased activity.

Trypsin inhibitor is soluble in water and phosphate buffers at concentrations of 10 mg/ml or higher. Solutions at higher concentrations may be hazy and have a yellow to amber color.

Unit Definition

One trypsin unit will produce a ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.

Biochem/physiol Actions

大豆胰蛋白酶抑制剂可抑制胰蛋白酶活性，而很少抑制胰凝乳蛋白酶和纤溶酶活性。它可以 1:1 的化学计量与胰蛋白酶形成复合物。形成这种复合物后，胰蛋白酶可裂解抑制剂中的精氨酸-异亮氨酸单键。这种复合物的解离可生成改性或天然的抑制剂。在胰蛋白酶结合的最佳 pH 值 (pH 8.0) 下，缔合常数 ≥ 10×10⁸。