Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Other Notes

Contains chromatographically purified soybean trypsin inhibitor and iron supplemented calf serum.

查看更多关于 胰蛋白酶抑制剂的信息。

Preparation Note

Prepared in Dulbecco′s Phosphate Buffered Saline without calcium and magnesium.

胰蛋白酶抑制剂可以 10mg/mL 或更高的浓度溶于水和磷酸盐缓冲液。浓度较高时溶液可能变浑浊，呈黄色至琥珀色。

Unit Definition

One trypsin unit = A₂₅₃ of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C.

Application

Optimized for passage of endothelial cell cultures.
Trypsin inhibitor from Glycine max (soybean) was used for the following applications:
? Filteration and seeding of dispersed acini
? Relative quantification of phospho-tau and immunoblotting analysis
? Subcloning and transformation of VIL-4 and IL-4 cDNA, and protein expression and purification (trypsin inhibitor form glycine max is used as positive control).

Biochem/physiol Actions

大豆胰蛋白酶抑制剂可抑制胰蛋白酶活性，而很少抑制胰凝乳蛋白酶和纤溶酶活性。它可以 1:1 的化学计量与胰蛋白酶形成复合物。形成这种复合物后，胰蛋白酶可裂解抑制剂中的精氨酸-异亮氨酸单键。这种复合物的解离可生成改性或天然的抑制剂。在胰蛋白酶结合的最佳 pH 值 (pH 8.0) 下，缔合常数 ≥ 10×10⁸。