Other Notes

仅用于生命科学研究。不可用于诊断。

Preparation Note

Activator: Ca²⁺ (0.12 mM) in combination with Mg²⁺ enhances activity [Laskowski (1971); Melgar and Goldthwait (1968)].
Working concentration: 1 mg/ml
Do not vortex while dissolving!
Storage conditions (working solution): Reconstituted in water, the solution can be kept for 2 to 3 days at 2 to 8 °C. Do not vortex during dissolving. Note: Dissolve at least 1 mg/ml.

Unit Definition

One unit is the enzyme activity that causes an increase in the absorbance of 0.001 per minute under assay conditions.

Application

Isolation procedures for proteins (e.g., membrane proteins).
Bovine pancreatic deoxyribonuclease I (DNase I) has been used for-
? The isolation of cells from lung, skin and tumor samplesBacterial DNA extraction from live bacterial cells that are resistant to DNase I ?

General description

Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III. DNase I is one of the most well characterized endonucleases of mammalian origin. It is a double-strand-specific endonuclease that requires bivalent cations for maximal activity.