Analysis Note

Protein determined by biuret

Unit Definition

One unit will deaminate 1.0 μmole of L -phenylalanine to trans-cinnamate and NH₃ per min at pH 8.5 at 30 °C.

Physical form

Solution in 60% glycerol, 3 mM Tris-HCl, pH 7.5, containing up to 0.5 M (NH₄)₂SO₄

Application

Phenylalanine ammonia-lyase has been used in a study to assess the effect of heat treatment on lignification of postharvest bamboo shoots. It has also been used in a study to investigate the effect of light on gene expression and podophyllotoxin biosynthesis in Linum album cell culture.

Biochem/physiol Actions

Phenylalanine Ammonia-Lyase from Rhodotorula glutinis can be cleaved and inactivated by the proteases chymotrypsin, subtilisin, and trypsin.