Analysis Note

由缩二脲测定的蛋白质。

包装

100, 200, 1000 units in poly bottle

5000 units in glass bottle

Unit Definition

在 pH 7.4 和 25°C 下，一单位本品每分钟可将 1.0μmol 1,6-二磷酸果糖转化为磷酸二羟丙酮和甘油醛 3-磷酸。

Physical form

2.5M (NH₄)₂SO₄、0.01M Tris溶液中的结晶悬浮液，pH 7.5，含 0.001M EDTA

Application

Aldolase is used to convert fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Aldolase, from rabbit muscle has been used for stereospecific deprotonation at DHAP C3 .

Biochem/physiol Actions

Aldolase is involved in gluconeogenesis, the Calvin cycle and glycolysis. Aldolase, from rabbit muscle, is a class I aldolase which forms covalent Schiff base intermediates. The active site of aldolase is in the center of the α/β ⁸ barrel fold .