Application

在多种马铃薯普通栽培种中至少发现了两种同工酶：一种具有高 ATP 酶/ADP 酶比率（约 10），另一种该比率较低（约 1）。
反应：ATP → ADP+P_i → AMP+2P_i。

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1 Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation. The enzyme has been used to assess the possibility of ATP involvement in intestinal lamina propria Th17 cell differentiation.

Other Notes

高比率和低比率同工酶混合物。

包装

100, 200 units in glass bottle

Sold on the basis of ATPase units

500, 1000, 2000, 5000 units in poly bottle

Quality

Contains ≤ 5% acid phosphatase.

Unit Definition

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

Biochem/physiol Actions

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM. The molecular weight of the protein is found to be approximately 45 kDa.