EINECS 编号 232-909-5
MDL number MFCD00132092
eCl@ss 32160410
Popular Documents: Specification Sheet (PDF)
An unusually non-specific protease.
1, 5 g in glass bottle
100 mg in glass bottle
Collected from culture broth of S. griseus.
Contains approximately 25% calcium acetate.
A mixture of at least three proteolytic activities including an extracellular serine protease. In general, serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid.
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
Protease is typically used in nucleic acid isolation procedures in incubations of 0.5-3.0 hours supplemented with 0.2% sodium dodecyl sulfate and 10 mM EDTA.
This enzyme is more active at a higher pH range than the known alkaline protease, showing the proteolytic activity even in 0.2N NaOH solution. This enzyme is useful for proteolysis of insoluble protein and for structure investigation of protein.
Completely inactivated by heating above 80 °C for 15-20 minutes.
product line | BioReagent |
form | powder |
impurities | starch, essentially free |
suitability | suitable for mouse embryo cell culture |
storage temp. | ?20°C |
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