EINECS 编号 232-582-9
MDL number MFCD00130830
Popular Documents: Specification Sheet (PDF)
用于胶原蛋白的消化和底物中贴壁细胞的释放。
Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethy
也具有梭菌蛋白酶、非特异性中性蛋白酶和胰蛋白酶活性
As supplied, this product is stable for one year at -20°C. There is no loss in FALGPA or protease activity in 30 days at 37°C, 50°C and -20°C. Solutions of crude collagenase are stable if frozen quickly in aliquots (at 10 mg/mL) and kept frozen at -20°C. Further freeze-thaw cycles will damage the solution. The product retains 100% activity over 7 hours when held on ice.
This proudct is prepared from Type XI (C7657) Collagenase from Clostridium histolyticum. It also contains clostripain, nonspecific neutral protease and tryptic activities. Solutions are typically prepared at 1-2 mg/mL in TESCA buffer (containing 50 mM TES, 0.36 mM Calcium chloride, pH 7.4 at 37°C.
One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro
form | powder |
activity | ≥800 units/mg solid |
suitability | suitable for cell culture |
storage temp. | ?20°C |
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