Analysis Note

最佳 pH 值为 2-4。在 4M 脲和 3M 盐酸胍中具有活性。在 60°C 下能稳定存在。在 pH 8.0-8.5 下可对胃蛋白酶进行不可逆地灭活。

Application

用于产生抗体的 F(ab′)₂ 片段。

Biochem/physiol Actions

优先裂解：P1 和 P1′ 位的疏水性和芳族残基。降解胰岛素 β 链中的 Phe-Val、Gln-His、Glu-Ala、Ala-Leu、Leu-Tyr、Tyr-Leu、Gly-Phe、Phe-Phe 和 Phe-Tyr 键

Other Notes

Peptide synthesis, Review.

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Unit Definition

One unit corresponds to the amount of enzyme which increases the absorbance at 280 nm by 0.001 per minute at pH 2.0 and 37 °C (Hemoglobin, Catalog No. 51290, as substrate); 15,000 absorbance units as described above are equivalent to ～1 Bergmeyer unit. One Bergmeyer unit is the amount of enzyme which hydrolyzes 1 μmole Acetyl-L-phenyl-3,5-diiodo-L-tyrosine at pH 2.0 and 37 °C.