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Qiagen 凯杰 Biosharp Omega

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Clostripain from Clostridium histolyticum ≥20 units/mg solid

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号:C0888-250UN

牌:Sigma 西格玛

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EA (预计5-7工作日到货(以Sigma 西格玛官网货期为准))

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别名: Clostridiopeptidase B Proteinase from Clostridium histolyticum

产品名称

Analysis Note

The enzyme must be activated for 2-3 hours before use by dissolving in 2.5 mM dithiothreitol containing 1.0 mM calcium acetate.

包装

Bottomless glass bottle. Contents are inside inserted fused cone.

Quality

Purified, essentially salt-free

Unit Definition

One unit will hydrolyze 1.0 μmole of BAEE per min at pH 7.6 at 25 °C in the presence of 2.5 mM DTT.

Biochem/physiol Actions

Clostripain from Clostridium histolyticum is composed of two polypeptide chains, with molecular masses of 41.7 kDa and 15.4 kDa. Clostripain has a highly restricted substrate specificity for Arg-Xaa peptide bonds. Therefore, clostripain has been explored as a potential enzyme for protein sequencing purposes. It has also been studied as a catalyst for condensation of pharmaceutically important peptides containing Arg-Pro bonds.


性质

form   lyophilized powder
impurities   salt, essentially free
storage temp.   2-8°C

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