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Bromelain from pineapple stem 5-15 units/mg protein

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号:B5144-100UN

牌:Sigma 西格玛

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产品名称

包装

100, 250 units in glass bottle

Preparation Note

Chromatographically purified. Dissolves in water at 1 mg/mL concentration to form a clear, colorless solution.

色谱纯化产品

Unit Definition

One unit will release 1.0 μmole of p-nitrophenol from N-α-Z- L -lysine p-nitrophenyl ester per min at pH 4.6 at 25 °C.

Physical form

Lyophilized powder containing mannitol and potassium phosphate buffer salts

Application

Bromelain may be used to inhibit the biosysnthesis of proinflammatory prostaglandins. It may be used to reduce clotting efficiency. Bromelain, from pineapple stem, has been used to make enzymatic hydrolysates of honeybee-collected pollen .

Biochem/physiol Actions

Bromelain is a cysteine endopeptidase with broad specificity for cleavage of proteins. Bromelain may be from a stem or piece of fruit. Product P3169 (pGlu-Phe-Leu-p-nitroanilide) may be used as a chromogenic substrate for the assay of bromelain.


性质

form   lyophilized powder
composition   Protein, 30-50% biuret
solubility   H2O: soluble1.0 mg/mL, clear, colorless
storage temp.   ?20°C

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