EINECS 编号 232-599-1
MDL number MFCD00081597
Popular Documents: Specification Sheet (PDF)
Extinction Coefficient: E1% = 21.0 (280 nm)
pI: 9.0
100, 200, 1000 units in poly bottle
This enzyme is soluble in water (10 mg/ml), yielding a clear solution.
One unit will produce a ΔA280 of 1.0 per min at pH 7.0 at 37°C when measuring TCA soluble products from casein in a final volume of 10 ml (1 cm light path).
Lyophilized powder containing
Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanili
form | lyophilized powder |
activity | ≥1.0 units/mg protein |
mol wt | mol wt 23.8 kDa |
composition | Protein, 80-100% biuret |
storage temp. | ?20°C |
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