Application

用于产生抗体的 Fab 片段。因其对某些组织的作用效果更好、破坏性更低，因此还用于细胞解离。

Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies. It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules. Product P3375, papain crude powder, has been used to digest fresh cartilage of dogsfish in order to isolate chondroitin sulfate.

Biochem/physiol Actions

半胱氨酸蛋白酶，可裂解碱性氨基酸、亮氨酸或甘氨酸的肽键。
最佳 pH 值为 6.0-7.0
也可水解酯和酰胺。

Papain papaya latex has antifungal activity against C. albicans. It is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.

Other Notes

查看更多关于 木瓜酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer。

包装

1 kg in poly bottle

25, 100 g in poly bottle

Quality

Not standardized with lactose or other adulterants.