EINECS 编号 254-457-8
MDL number MFCD00132129
eCl@ss 32160410
Popular Documents: Datasheet (PDF) | Specification Sheet (PDF)
Frequently Asked Questions are available for this Product.
Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.
1 g in poly bottle
10, 25, 100, 500 mg in poly bottle
5 mg in glass bottle
One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.
PicoGreen 为以下机构的注册商标: Life Technologies
Quality Level | ELITE |
grade | for molecular biology |
product line | BioUltra |
form | lyophilized powder |
mol wt | mol wt 28.93 kDa |
composition | Protein, ≥90% biuret |
impurities | ≤500 pg/mg DNA (PicoGreen® assay) |
foreign activity | DNAse, Nickase and RNAse, none detected |
shipped in | wet ice |
storage temp. | ?20°C |
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