EINECS 编号 232-752-2
MDL number MFCD00132092
eCl@ss 32160410
Popular Documents: Specification Sheet (PDF)
Frequently Asked Questions are available for this Product.
Amino acid analysis and isoelectric focusing electrophoresis consistent with subtilisin Carlsberg.
1 g in glass bottle
50, 100, 250 mg in glass bottle
Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
The enzyme from Sigma has been used to optimize release of all mitochondrial populations from homogenized ventricular tissue of rat heart. It has also been used in the pre-hybridisation treatment of formalin fixed, paraffin wax-embedded liver specimens for detecting human and viral DNA.
This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. Product P8038, also known as Subtilisin Carlsberg, has been used to hydrolyze cardiac cells to study the silencing of cardiac mitochondrial NHE1.
Proteinase catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphat
Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.
type | Type XXIV |
form | lyophilized powder |
activity | ≤0.05 Kunitz units/mg solid RNase |
≤5.0 Kunitz units/mg solid DNase | |
purified by | crystallization |
shipped in | wet ice |
storage temp. | ?20°C |
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