Analysis Note

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.

基于 Biggs 改性法的 NIH 分析过程使用 0.2mL 稀释血浆（由血浆与等量生理盐水混合制得）作为底物，以及 0.1mL 凝血酶样品（在含 1% 白蛋白缓冲液中保持稳定）。只使用 15-25 秒之间的凝血时间分析凝血酶浓度。

Biochem/physiol Actions

丝氨酸蛋白酶，选择性断开纤维蛋白原中的精氨酸-甘氨酸键，形成纤维蛋白以及纤维蛋白肽 A 和 B。

General description

Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.

Other Notes

查看更多关于 凝血酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer。

Preparation Note

Traditional thrombin products are activated with bovine brain, whereas T7201 is activated with bovine lung and does not contain any bovine brain products.

Physical form

Lyophilized from saline sodium citrate buffer, pH 6.5

Application

Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess a novel antiplatelet therapy in acute coronary syndromes.