Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Analysis Note

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.

基于 Biggs 改性法的 NIH 分析过程使用 0.2mL 稀释血浆（由血浆与等量生理盐水混合制得）作为底物，以及 0.1mL 凝血酶样品（在含 1% 白蛋白缓冲液中保持稳定）。只使用 15-25 秒之间的凝血时间分析凝血酶浓度。

Biochem/physiol Actions

丝氨酸蛋白酶，选择性断开纤维蛋白原中的精氨酸-甘氨酸键，形成纤维蛋白以及纤维蛋白肽 A 和 B。

General description

Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.

Other Notes

查看更多关于 凝血酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer。

包装

1000, 10000, 100000 units in glass bottle

Preparation Note

Activated with bovine brain thromboplastin.

Physical form

Lyophilized powder containing sodium chloride and Tris-HCl, pH 7.0

Application

Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess persistent hypocoagulability in patients with septic shock.