EINECS 编号 254-453-6
MDL number MFCD00130998
Popular Documents: Product Information Sheet (PDF) | Specification Sheet (PDF)
Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.
Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9 kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester. The pH optimum is found to be 8.0-8.5. It does not require any activator, but it is inhibited by diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride, α2-macroglobulin, α1-antitrypsin, sulfonyl fluorides, p-dinitrophenyl diethylphosphate and high salt concentrations. Elastase is extensively used in tissue and cell dissociation procedures. It is effective in the isolation of Type II lung cells.
10, 20 mg in poly bottle
5 mg in glass bottle
Package size based on protein content
100 mg in glass bottle
2x crystallized and chromatographically purified
Contains trypsin activity
在 pH 8.0 和 25°C 下,一单位每分钟可水解 1.0μmol 的 N-琥珀酰-
含有碳酸钠。
Elastase from porcine pancreas has been used in a study to identify a novel bone/calcium metabolism-regulating factor in porcine pancreas. Elastase from porcine pancreas has also been used in a study to investigate the effect of a specific synthetic inhibitor of neutrophil elastase (ONO-5046) on the course of acute hemorrhagic pancreatitis in dogs.
The enzyme from Sigma has been used in the proteolytic cleavage of UMP synthase (Uridine-5′-monophosphate synthase). It has also been used along with collagenase to disperse tissue into single cells from the thoracic media, abdominal aortic media and intima of rabbit.
type | Type III |
form | lyophilized powder |
composition | Protein, 55-85% |
solubility | 200 mM Tris HCl buffer, pH 8.8: soluble1.0 mg/mL, clear |
storage temp. | ?20°C |
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