EINECS 编号 232-671-2
MDL number MFCD00130481
eCl@ss 42010112
Popular Documents: Specification Sheet (PDF)
Frequently Asked Questions are available for this Product.
Protein determined by E
一种丝氨酸蛋白酶,能将与芳香烃或其他大型疏水性侧链
α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10 mM Cu2+ and Hg2+.
View more information on chymotrypsin at www.sigma-aldrich.com/enz
1, 10 g in poly bottle
250, 500 mg in poly bottle
Produced from 3× crystallized chymotrypsinogen
One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.
The enzyme from Sigma has been used to study the structure-function relationship in glycosylated α-chymotrypsin using immobilized metal-ion affinity chromatography (IMAC) and immobilized metal-ion affinity capillary electrophoresis (IMACE).
Quality Level | PREMIUM |
type | Type II |
form | lyophilized powder |
mol wt | mol wt 25 kDa |
composition | protein, ≥85% |
color | white to off-white |
Featured Industry | Diagnostic Assay Manufacturing |
storage temp. | ?20°C |
Gene Information | cow ... CTRB1(618826) |
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