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TAMRA Azide is a copper (I)-catalyzed azide-alkyne cycloaddition (CuAAC) reagent of bright yellow dye that used to generate a stable fluorescence signal in bioimaging. The maxima of Ex/Em values are at 543/575 nm, similar to that of DyLight 549, ATTO 550 and Cy 3. TAMRA might be excited using 543 or 546 nm laser line and displays good optical property. The azide reactive group is connected to the dye through an amino propyl linkage. TAMRA azide couples with an alkyne to form 1,4-disubstituted 1,2,3-triazole inside of living systems without interfering native biochemical processes. Prior to perform CuAAC, the alkyne functionality should be introduced onto counterpart biomolecule by means of chemical or genetic modification. We offer TAMRA Azide as a click chemistry reagent dye for cellular imaging and nucleotide functionalization.