Anti-BACE, C-terminus, clone 61-3E7 (mouse monoclonal) - 炼石商城

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Anti-BACE, C-terminus, clone 61-3E7 (mouse monoclonal)

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货号：MAB5308

品牌：Millipore 密理博

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R, Pm	IP, WB	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MAB5308
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-BACE Antibody, CT, clone 61-3E7
Alternate Names	ASP2 BACE1 Beta Secretase beta-Site APP Cleaving Enzyme
Background Information	Alzheimer's disease (AD) is characterized by the progressive formation in the brain of insoluble amyloid plaques and vascular deposits consisting of the 4-kD amyloid b-peptide (Ab). Ab generation is initiated by proteolytic cleavage of the amyloid precursor protein (APP) at the N-terminal of Ab by b-secretase. The Ab peptide is then released by proteolytic cleavage at its C-terminus by g-secretase. Because both these proteases are prime candidates for therapeutic intervention, an intense search has been underway to identify these two enzymes.A human transmembrane aspartic-protease (Asp2), referred to as BACE, has been characterized and shown to have all the properties of b-secretase. Four groups in all have now confirmed that BACE (or Asp2) is a convincing candidate for b-secretase. BACE is an N-glycosylated integral membrane aspartyl protease with Mr=70 kDa. Mature BACE is produced from the immature form through a series of post-translational proteolytic cleavages and glycosylation. Sequence analysis has revealed that the immature form of BACE contains an N-terminal signal sequence (residues 1-21) followed by a large catalytic domain, a single transmembrane domain (residues 461-477), and a short cytoplasmic domain (residues 478-501). The signal sequence (1-21) is cleaved from the immature form by a signal peptidase located in the endoplasmic reticulum (ER), yielding the proBACE protein (Mr=75 kDa) which starts at residue 22. The proBACE protein is modified by cleavage of 24 N-terminal residues (aa 22-45), producing the mature BACE protein.

References

Product Information
Format	Purified
Control	Brain
Presentation	Purified immunoglobulin. Liquid. Buffer = 0.02M Sodium Phosphate, 0.25M NaCl with 0.1% sodium azide.

Applications
Application	Detect BACE using this Anti-BACE Antibody, C-terminus, clone 61-3E7 validated for use in IP & WB.
Key Applications	Immunoprecipitation Western Blotting
Application Notes	Western blotting: 1 μg/mL; recognizes pro and mature forms: ~60-75kDa on reducing westerns. BACE is N-terminally glycosylated which causes the wide size range. Immunohistochemistry on paraformaldehyde fixed tissues from human, rat, mouse and monkey. Immunocytochemistry on cells expressing BACE. Acetone or methanol fixation preferred; 4% PFA 5', RT followed by 0.1% triton X-100 1 hour, can also be used. 1:200-1:500 is recommended, optimization is necessary. Immunoprecipitation: Optimal working dilutions must be determined by end user.

Biological Information
Immunogen	Synthetic peptide corresponding to the C-terminus of human BACE.
Epitope	C-terminus
Clone	61-3E7
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	Reacts with BACE (beta-site APP Cleaving Enzyme). Shows no reactivity to BACE2 by Western blot.
Isotype	IgG1
Species Reactivity	Human Mouse Rat Primate
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_012104.3 NM_138971.2 NM_138972.2 NM_138973.2
Entrez Gene Summary	Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is the protein encoded by this gene. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Four transcript variants encoding different isoforms have been described for this gene.
Gene Symbol	BACE1 ASP2 beta-secretase KIAA1149 Memapsin-2 BACE memapsin-2 HSPC104 FLJ90568 EC 3.4.23.46
Purification Method	Protein A Purfied
UniProt Number	P56817
UniProt Summary	FUNCTION: SwissProt: P56817 # Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. SIZE: 501 amino acids; 55764 Da SUBUNIT: Monomer. Interacts with GGA1, GGA2 and GGA3. Interacts with RTN3 and RTN4. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Brain. SIMILARITY: SwissProt: P56817 ## Belongs to the peptidase A1 family.
Molecular Weight	~ 60-75 kDa

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Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Packaging Information
Material Size	100 µg

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