Ms X Calretinin

Species Reactivity	Key Applications	Host	Format	Antibody Type
M, R	IHC, WB	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

M, R

IHC, WB

Purified

Monoclonal Antibody

Description
Catalogue Number	MAB1568
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Calretinin Antibody, clone 6B8.2
Alternate Names	calbindin 2 calbindin 2, (29kD, calretinin)
Background Information	Calretinin is a 29 kDa calcium binding protein that is expressed in central and peripheral nervous system and in many normal and pathological tissues. Calretinin is also considered an important diagnostic aid in the differential diagnosis of cystic and solid ameloblastic tumors. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Calretinin is generally expressed with two other calcium binding proteins Calbindin D-29 and parvalbumin in CNS and other peripheral tissues. Calretinin antibodies have been used to detect a number of carcinomas by clinicopathological, immunohistochemical techniques including, epithelial malignant mesotheliomas, Ovarian yolk sac tumor, Adenomatoid tumors of the uterus etc

Description

Catalogue Number

MAB1568

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Calretinin Antibody, clone 6B8.2

Alternate Names

calbindin 2
calbindin 2, (29kD, calretinin)

Background Information

Calretinin is a 29 kDa calcium binding protein that is expressed in central and peripheral nervous system and in many normal and pathological tissues. Calretinin is also considered an important diagnostic aid in the differential diagnosis of cystic and solid ameloblastic tumors. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Calretinin is generally expressed with two other calcium binding proteins Calbindin D-29 and parvalbumin in CNS and other peripheral tissues. Calretinin antibodies have been used to detect a number of carcinomas by clinicopathological, immunohistochemical techniques including, epithelial malignant mesotheliomas, Ovarian yolk sac tumor, Adenomatoid tumors of the uterus etc

Product Information
Format	Purified
Control	Rat brain cortex, Mesothelioma tissue, mouse brain lysate.
Presentation	Purified mouse monoclonal IgG1 in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.

Product Information

Format

Purified

Control

Rat brain cortex, Mesothelioma tissue, mouse brain lysate.

Presentation

Purified mouse monoclonal IgG1 in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.

Applications
Application	Anti-Calretinin Antibody, clone 6B8.2 detects level of Calretinin & has been published & validated for use in IH & WB.
Key Applications	Immunohistochemistry Western Blotting
Application Notes	Immunohistochemistry: On rat brain tissue: IFA: 1:1,000-1:5,000 PAP: 1:1,000-1:5,000 The antibody has been used successfully on formalin-fixed, paraffin embedded human tissue after pre-treatment of the tissue slides with citrate buffer and heat treatment. Suggested starting dilution 1:100-1:400. Proteolytic digestion with pepsin is detrimental to the immunostaining. Western blot: 1:1,000-1:3,000 Optimal working dilutions must be determined by the end user.

Applications

Application

Anti-Calretinin Antibody, clone 6B8.2 detects level of Calretinin & has been published & validated for use in IH & WB.

Key Applications

Immunohistochemistry
Western Blotting

Application Notes

Immunohistochemistry:
On rat brain tissue:
IFA: 1:1,000-1:5,000
PAP: 1:1,000-1:5,000
The antibody has been used successfully on formalin-fixed, paraffin embedded human tissue after pre-treatment of the tissue slides with citrate buffer and heat treatment. Suggested starting dilution 1:100-1:400. Proteolytic digestion with pepsin is detrimental to the immunostaining.

Western blot:
1:1,000-1:3,000

Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	Recombinant rat calretinin (Heinze, L., 2007).
Clone	6B8.2
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	Calretinin is a neuron specific calcium binding protein (31 kDa) found mostly in the CNS and retina. It is present in subsets of neurons throughout the brain and spinal cord, including sensory ganglia. It shares about 55% amino acid homology with calbindin, another calcium binding protein found in brain and periphery. Studies suggest that calretinin, like calbindin, may be neuroprotective.
Isotype	IgG1
Species Reactivity	Mouse Rat
Species Reactivity Note	Mouse and rat. Other species have not been tested.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_001740.3
Entrez Gene Summary	Calbindin 2 (calretinin), closely related to calbindin 1, is an intracellular calcium-binding protein belonging to the troponin C superfamily. Calbindin 1 is known to be involved in the vitamin-D-dependent calcium absorption through intestinal and renal epithelia, while the function of neuronal calbindin 1 and calbindin 2 is poorly understood. The sequence of the calbindin 2 cDNA reveals an open reading frame of 271 codons coding for a protein of 31,520 Da, and shares 58% identical residues with human calbindin 1. Calbindin 2 contains five presumably active and one presumably inactive calcium-binding domains. Comparison with the partial sequences available for chick and guinea pig calbindin 2 reveals that the protein is highly conserved in evolution. The calbindin 2 message was detected in the brain, while absent from heart muscle, kidney, liver, lung, spleen, stomach and thyroid gland. There are two additional forms of alternatively spliced calbindin 2 mRNAs encoding C-terminally truncated proteins. Exon 7 can splice to exon 9, resulting in a frame shift and a translational stop at the second codon of exon 9, and encoding calretinin-20k. Exon 7 can also splice to exon 10, resulting in a frame shift and a translational stop at codon 15 of exon 10, and encoding calretinin-22k. The truncated proteins are able to bind calcium.
Gene Symbol	CALB2 Calretinin CAB29 CR CAL2
Purification Method	Protein A Purfied
UniProt Number	P22676
UniProt Summary	FUNCTION: SwissProt: P22676 # Calretinin is a calcium-binding protein which is abundant in auditory neurons. SIZE: 271 amino acids; 31540 Da TISSUE SPECIFICITY: Brain. SIMILARITY: SwissProt: P22676 ## Belongs to the calbindin family. & Contains 6 EF-hand domains.
Molecular Weight	31 kDa

Biological Information

Immunogen

Recombinant rat calretinin (Heinze, L., 2007).

Clone

6B8.2

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

Calretinin is a neuron specific calcium binding protein (31 kDa) found mostly in the CNS and retina. It is present in subsets of neurons throughout the brain and spinal cord, including sensory ganglia. It shares about 55% amino acid homology with calbindin, another calcium binding protein found in brain and periphery. Studies suggest that calretinin, like calbindin, may be neuroprotective.

Isotype

IgG1

Species Reactivity

Mouse
Rat

Species Reactivity Note

Mouse and rat. Other species have not been tested.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_001740.3

Entrez Gene Summary

Calbindin 2 (calretinin), closely related to calbindin 1, is an intracellular calcium-binding protein belonging to the troponin C superfamily. Calbindin 1 is known to be involved in the vitamin-D-dependent calcium absorption through intestinal and renal epithelia, while the function of neuronal calbindin 1 and calbindin 2 is poorly understood. The sequence of the calbindin 2 cDNA reveals an open reading frame of 271 codons coding for a protein of 31,520 Da, and shares 58% identical residues with human calbindin 1. Calbindin 2 contains five presumably active and one presumably inactive calcium-binding domains. Comparison with the partial sequences available for chick and guinea pig calbindin 2 reveals that the protein is highly conserved in evolution. The calbindin 2 message was detected in the brain, while absent from heart muscle, kidney, liver, lung, spleen, stomach and thyroid gland. There are two additional forms of alternatively spliced calbindin 2 mRNAs encoding C-terminally truncated proteins. Exon 7 can splice to exon 9, resulting in a frame shift and a translational stop at the second codon of exon 9, and encoding calretinin-20k. Exon 7 can also splice to exon 10, resulting in a frame shift and a translational stop at codon 15 of exon 10, and encoding calretinin-22k. The truncated proteins are able to bind calcium.

Gene Symbol

CALB2
Calretinin
CAB29
CR
CAL2

Purification Method

Protein A Purfied

UniProt Number

P22676

UniProt Summary

FUNCTION: SwissProt: P22676 # Calretinin is a calcium-binding protein which is abundant in auditory neurons.
SIZE: 271 amino acids; 31540 Da
TISSUE SPECIFICITY: Brain.
SIMILARITY: SwissProt: P22676 ## Belongs to the calbindin family. & Contains 6 EF-hand domains.

Molecular Weight

31 kDa

Product Usage Statements
Quality Assurance	Routinely evaluated by Western Blot on mouse brain lysates. Western Blot Analysis: 1:1000 dilution of this lot detected Calretinin on 10 μg of mouse brain lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by Western Blot on mouse brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected Calretinin on 10 μg of mouse brain lysates.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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