Anti-CD42a, Platelet lb-IX complex, cln FMC-25 (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	FC, ICC, IHC	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

FC, ICC, IHC

Purified

Monoclonal Antibody

Description
Catalogue Number	MAB1202
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-CD42a Antibody, platelet lb-IX, clone FMC-25

Description

Catalogue Number

MAB1202

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-CD42a Antibody, platelet lb-IX, clone FMC-25

Product Information
Format	Purified
Presentation	Purified from mouse ascitic fluid. The antibody is supplied in 25mM Tris-HCl, 0.4M NaCl, pH 8.0 containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry.

Product Information

Format

Purified

Presentation

Purified from mouse ascitic fluid. The antibody is supplied in 25mM Tris-HCl, 0.4M NaCl, pH 8.0 containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry.

Applications
Application	Anti-CD42a Antibody, platelet lb-IX, clone FMC-25 detects level of CD42a & has been published & validated for use in FC, IC, IH.
Key Applications	Flow Cytometry Immunocytochemistry Immunohistochemistry
Application Notes	The glycoprotein Ib-IX complex on the surface of normal platelets is a heterotrimer of Ib-alpha, Ib-beta and IX subunits (Berndt, 1989). Ib-IX complex is an essential component of platelet adhesion to vascular subendothelium and is also important in the maintenance of platelet shape (Berndt et al., 1988). This antibody is suitable for enumeration of platelets by flow cytometry and immunoperoxidase staining of frozen tissue sections or air dried smear preparations (San Miguel et al., 1986), and for identification of megakaryoblasts in acute myeloid leukemia or acute myelofibrosis (San Miguel et al., 1985; San Miguel et al., 1986). SUGGESTED USAGE DILUTION 1. Flow cytometry and indirect immunofluorescence 1:25 Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 platelets in 100 μl of buffer. 2. Indirect immunoperoxidase staining - the final dilution will depend on the assay conditions and detection system employed. However, a dilution of at least 1:20 will be applicable to most commonly used systems.

Applications

Application

Anti-CD42a Antibody, platelet lb-IX, clone FMC-25 detects level of CD42a & has been published & validated for use in FC, IC, IH.

Key Applications

Flow Cytometry
Immunocytochemistry
Immunohistochemistry

Application Notes

The glycoprotein Ib-IX complex on the surface of normal platelets is a heterotrimer of Ib-alpha, Ib-beta and IX subunits (Berndt, 1989). Ib-IX complex is an essential component of platelet adhesion to vascular subendothelium and is also important in the maintenance of platelet shape (Berndt et al., 1988).

This antibody is suitable for enumeration of platelets by flow cytometry and immunoperoxidase staining of frozen tissue sections or air dried smear preparations (San Miguel et al., 1986), and for identification of megakaryoblasts in acute myeloid leukemia or acute myelofibrosis (San Miguel et al., 1985; San Miguel et al., 1986).

SUGGESTED USAGE DILUTION

1. Flow cytometry and indirect immunofluorescence 1:25

Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 platelets in 100 μl of buffer.

2. Indirect immunoperoxidase staining - the final dilution will depend on the assay conditions and detection system employed. However, a dilution of at least 1:20 will be applicable to most commonly used systems.

Biological Information
Immunogen	Peripheral blood mononuclear cells isolated on Ficoll™ hypaque.
Epitope	platelet lbalpha complex
Clone	FMC-25
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody belongs to CD42a (assigned at the Fourth International Workshop on Leukocyte Differentiation Antigens, Vienna, 1989) (Knapp, 1989), and binds to the 22,000 dalton IX subunit of the glycoprotein Ib-IX complex (Zola et al., 1984; Berndt et al., 1985a,b). FMC-25 failed to bind to platelets from patients with Bernard-Soulier Syndrome (Berndt et al., 1983). The antibody is specific for cells of the platelet megakaryocyte lineage. It reacts with a proportion of acute myeloid leukemias being useful in identifying those of the megakaryoblastic subtype (San Miguel et al., 1985, 1986). Cell reactivity: The antibody is specific for cells of the platelet megakaryocyte lineage. It reacts with a proportion of acute myeloid leukemias being useful in identifying those of the megakaryoblastic subtype(San Miguel et al., 1985, 1986).
Isotype	IgG1
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_000174.2
Entrez Gene Summary	Platelet glycoprotein IX (GP9) is a small membrane glycoprotein found on the surface of human platelets. It forms a 1-to-1 noncovalent complex with glycoprotein Ib (GP Ib), a platelet surface membrane glycoprotein complex that functions as a receptor for von Willebrand factor (VWF; MIM 193400). The main portion of the receptor is a heterodimer composed of 2 polypeptide chains, an alpha chain (GP1BA; MIM 606672) and a beta chain (GP1BB; MIM 138720), that are linked by disulfide bonds. The complete receptor complex includes noncovalent association of the alpha and beta subunits with GP9 and platelet glycoprotein V (GP5; MIM 173511).[supplied by OMIM]
Gene Symbol	GP9 CD42a GPIX
UniProt Number	P14770
UniProt Summary	FUNCTION: SwissProt: P14770 # The GPIb-V-IX complex functions as the von Willebrand factor receptor and mediates von willebrand factor-dependent platelet adhesion to blood vessels. The adhesion of platelets to injured vascular surfaces in the arterial circulation is a critical initiating event in hemostasis. GP-IX may provide for membrane insertion and orientation of GP-Ib. SIZE: 177 amino acids; 19046 Da SUBUNIT: GP-Ib alpha and beta are disulfide linked. GP-IX is complexed with the GP-Ib heterodimer via a non covalent linkage. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. DISEASE:SwissProt: P14770 # Defects in GP9 are a cause of Bernard-Soulier syndrome (BSS) [MIM:231200]; also known as giant platelet disease (GPD). BSS patients have unusually large platelets and have a clinical bleeding tendency. SIMILARITY: SwissProt: P14770 ## Contains 1 LRR (leucine-rich) repeat. MISCELLANEOUS: Platelet activation apparently involves disruption of the macromolecular complex of GP-Ib with the platelet glycoprotein IX (GP-IX) and dissociation of GP-Ib from the actin- binding protein.

Biological Information

Immunogen

Peripheral blood mononuclear cells isolated on Ficoll™ hypaque.

Epitope

platelet lbalpha complex

Clone

FMC-25

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

This antibody belongs to CD42a (assigned at the Fourth International Workshop on Leukocyte Differentiation Antigens, Vienna, 1989) (Knapp, 1989), and binds to the 22,000 dalton IX subunit of the glycoprotein Ib-IX complex (Zola et al., 1984; Berndt et al., 1985a,b). FMC-25 failed to bind to platelets from patients with Bernard-Soulier Syndrome (Berndt et al., 1983). The antibody is specific for cells of the platelet megakaryocyte lineage. It reacts with a proportion of acute myeloid leukemias being useful in identifying those of the megakaryoblastic subtype (San Miguel et al., 1985, 1986).

Cell reactivity:

The antibody is specific for cells of the platelet megakaryocyte lineage. It reacts with a proportion of acute myeloid leukemias being useful in identifying those of the megakaryoblastic subtype(San Miguel et al., 1985, 1986).

Isotype

IgG1

Species Reactivity

Human

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_000174.2

Entrez Gene Summary

Platelet glycoprotein IX (GP9) is a small membrane glycoprotein found on the surface of human platelets. It forms a 1-to-1 noncovalent complex with glycoprotein Ib (GP Ib), a platelet surface membrane glycoprotein complex that functions as a receptor for von Willebrand factor (VWF; MIM 193400). The main portion of the receptor is a heterodimer composed of 2 polypeptide chains, an alpha chain (GP1BA; MIM 606672) and a beta chain (GP1BB; MIM 138720), that are linked by disulfide bonds. The complete receptor complex includes noncovalent association of the alpha and beta subunits with GP9 and platelet glycoprotein V (GP5; MIM 173511).[supplied by OMIM]

Gene Symbol

GP9
CD42a
GPIX

UniProt Number

P14770

UniProt Summary

FUNCTION: SwissProt: P14770 # The GPIb-V-IX complex functions as the von Willebrand factor receptor and mediates von willebrand factor-dependent platelet adhesion to blood vessels. The adhesion of platelets to injured vascular surfaces in the arterial circulation is a critical initiating event in hemostasis. GP-IX may provide for membrane insertion and orientation of GP-Ib.
SIZE: 177 amino acids; 19046 Da
SUBUNIT: GP-Ib alpha and beta are disulfide linked. GP-IX is complexed with the GP-Ib heterodimer via a non covalent linkage.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DISEASE:SwissProt: P14770 # Defects in GP9 are a cause of Bernard-Soulier syndrome (BSS) [MIM:231200]; also known as giant platelet disease (GPD). BSS patients have unusually large platelets and have a clinical bleeding tendency.
SIMILARITY: SwissProt: P14770 ## Contains 1 LRR (leucine-rich) repeat.
MISCELLANEOUS: Platelet activation apparently involves disruption of the macromolecular complex of GP-Ib with the platelet glycoprotein IX (GP-IX) and dissociation of GP-Ib from the actin- binding protein.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Store at 2 to 8°C, for up to 6 months. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES. WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

Storage and Shipping Information

Storage Conditions

Store at 2 to 8°C, for up to 6 months. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-CD42a, Platelet lb-IX complex, cln FMC-25 (mouse monoclonal)

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