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Ms X SSEA-1 Alexa Fluor?488

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号:MAB4301X

牌:Millipore 密理博

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EA (预计3-5工作日到货)

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Replacement Information

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, R FC, ICC, IF M AlexaFluor®488 Monoclonal Antibody
Description
Catalogue Number MAB4301X
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480, Alexa Fluor® 488
Alternate Names
  • SSEA-1
Background Information SSEA-1 is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.
References
Product Information
Format AlexaFluor®488
Control
  • P19 mouse embryonic carcinoma cells
Presentation Purified immunoglobulin conjugated to Alexa Fluor® 488. Liquid in Phosphate buffer with 15 mg/mL BSA as a stabilizer and 0.1% sodium azide
Applications
Application This Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480, Alexa Fluor 488 is validated for use in FC, IC, IF for the detection of Stage-Specific Embryonic Antigen-1.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
  • Immunofluorescence
Application Notes FACS

Immunocytochemistry:

1. Fix cells with a 4% Paraformaldehyde solution for 30-45 minutes, and then rinse several times with Tris-Buffered-Saline (TBS).

2. Incubate cells with MAB4301X diluted to 1/100 with PBS/ Tween-20 (0.05%) / BSA (1%) for 30 minutes.

3. Wash the sample three times with PBS / Tween-20 (0.05%) for 1 minute per wash. Examine under a microscope fitted with a FITC filter.

Optimal working dilutions must be determined by end user.
Biological Information
Immunogen F9 teratocarcinoma stem cells (X-irradiated)
Clone MC-480
Host Mouse
Specificity Reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.
Isotype IgM
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary FUT4 catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). It has been detected in human embryos (5-10 weeks) suggesting a role in development.
Gene Symbol
  • FUT4
  • ELFT
  • FCT3A
  • Fuc-TIV
  • 1,3fucosyltransferase
  • FUC-TIV
  • FucT-IV
  • CD15
  • Alpha-
  • EC 2.4.1.-
Purification Method Ammonium Sulfate Precipitation
UniProt Number
UniProt Summary FUNCTION: SwissProt: P22083 # May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.
SIZE: 405 amino acids; 45570 Da
SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi.
SIMILARITY: SwissProt: P22083 ## Belongs to the glycosyltransferase 10 family.
Stem Cell Type
  • Human Embryonic Stem Cells
  • Mouse Embryonic Stem Cells
  • Neural Stem Cells
  • Induced Pluripotent Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain refrigerated at 2–8°C protected from light in undiluted aliquots for up to 24 months.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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