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Ms X SSEA-4

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货号：MAB4304

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M FC, ELISA, IF, IHC M Purified Monoclonal Antibody
Description
Catalogue Number MAB4304
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Stage-Specific Embryonic Antigen-4 Antibody, clone MC-813-70
Alternate Names
  • SSEA-4
Background Information The cell-surface markers used routinely to identify undifferentiated human embryonic stem cells (hESCs) were initially characterized as markers for mESCs, mouse embryonic carcinomas (ECs), or human EC cells. Stage-specific embryonic antigen-3 (SSEA-3) and SSEA-4 are widely used as cell-surface markers to define both human and mouse ESCs. SSEA-3 and SSEA-4 are expressed on undifferentiated hESCs but not on undifferentiated mESCs.
References
Product Information
Format Purified
Control
  • Pluripotent human embryonic stem (ES) cells
Presentation Purified moust monoclonal IgG3 liquid in buffer containing 0.02 M PBS, 0.25 M NaCl, pH 7.6 with 0.1% Sodium Azide
Applications
Application Anti-Stage-Specific Embryonic Antigen-4 Antibody, clone MC-813-70 is an antibody against Stage-Specific Embryonic Antigen-4 for use in FC, ELISA, IF, IH.
Key Applications
  • Flow Cytometry
  • ELISA
  • Immunofluorescence
  • Immunohistochemistry
Application Notes Immunohistochemistry: A previous lot of this antibody was used in IH.

Immunofluorescence: A previous lot of this antibody was used in IF.

ELISA: A previous lot of this antibody was used in ELISA.

Flow Cytometry: A starting range of 10-20 µg/mL is suggested.

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Human embryonal carcinoma cell line 2102Ep
Clone clone MC-813-70
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity This antibody reacts with the Stage-specific embryonic antigen-4 (SSEA-4) that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-4 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression.
Isotype IgG3
Species Reactivity
  • Human
  • Mouse
Antibody Type Monoclonal Antibody
Gene Symbol
  • SSEA-4
  • SSEA4
Purification Method Protein A Purfied
Stem Cell Type
  • Human Embryonic Stem Cells
  • Mouse Embryonic Stem Cells
  • Induced Pluripotent Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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