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Ms X VIN-IS-56 [GD3]

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货号：MAB4308

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
Ma FC, ICC, IHC M Purified Monoclonal Antibody
Description
Catalogue Number MAB4308
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-VIN-IS-56 Antibody, clone VIN-IS-56
Background Information Almost all melanomas, astrocytomas,a proportion of sarcomas, a small number of carcinomas, some nevi, as well as normal melanocytes express GD3 antigen.
GD3 is one of the most important markers for malignant melanoma. Antibodies to the GD3 ganglioside can induce partial remission of tumor growth in animals as well as in humans via enhancement of cytotoxic and proliferative response of lymphocytes.
Anti-GD3 antibodies have been found in association with specific forms of Guillain-Barre syndrome. In vivo studies of isolated anti-GM1 and GD3 antibodies indicate the antibodies can interfere with motor neuron function.
References
Product Information
Format Purified
Control
  • Undifferentiated human embryonic stem or embryonic carcinoma cells stain weakly
Presentation Purified immunoglobulin. Liquid in 0.05M Potassium Phosphate, 0.3M NaCl pH 8.0 with 0.05% Sodium Azide
Applications
Application Anti-VIN-IS-56 Antibody, clone VIN-IS-56 detects level of VIN-IS-56 & has been published & validated for use in FC, IC, IH.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
  • Immunohistochemistry
Application Notes Immunohistochemistry & Immunocytochemistry:

Suggested fixatives: Paraformaldehyde is recommended, NOT methanol or acetone (the glycolipid antigen is extracted by polar lipid solvents).

Recommended antibody dilution: 10-20 μg/ml

Dilution Buffer: PBS + 4% FCS

Expected staining pattern: cell surface staining



FACS Analysis:

Suggested dilution/number of cells: 10-20 μg/ml for 1 x 105 cells

Fixation/Permeabilization used: Live cells or fixed in 4% Paraformaldehyde

Recommended controls: Undifferentiated human ES or EC cells stain weakly.



Immunoblotting:

Not tested



EIA:

Not tested



Optimal working dilutions must be determined by end user.
Biological Information
Immunogen Human Embryonal carcinoma cells
Clone VIN-IS-56
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity This monoclonal antibody recognizes a ganglioseries glycolipid, gangliosides GD3. Experimental evidence suggests that the expression of this antigen increases following human Embryonal Carcinoma and Embryonic Stem cell differentiation. It is thought that this antibody recognizes cells of the neuroectoderm lineage.
Isotype IgM
Species Reactivity
  • Mammals
Antibody Type Monoclonal Antibody
Gene Symbol
  • VIN-IS-56
Stem Cell Type
  • Neural Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain refrigerated at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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