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Ms X TRA-1-81

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货号：MAB4381

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB, FC, IF, IP, ICC M Purified Monoclonal Antibody
Description
Catalogue Number MAB4381
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-TRA-1-81 Antibody, clone TRA-1-81
Background Information Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). Embryonal carcinoma, as do all human pluripotent stem cells, expresses TRA-1-60 and TRA-1-81 antigens, and although their molecular identities are unknown, they are commonly used as markers of undifferentiated pluripotent human stem cells.
References
Product Information
Format Purified
Control
  • Human embryonic stem cells
Presentation Liquid in 0.05 M Potassium Phosphate, 0.3 M NaCl, pH 8.0 with 0.05% Sodium Azide.
Applications
Application Anti-TRA-1-81 Antibody, clone TRA-1-81 detects level of TRA-1-81 & has been published & validated for use in WB, FC, IF, IP & IC.
Key Applications
  • Western Blotting
  • Flow Cytometry
  • Immunofluorescence
  • Immunoprecipitation
  • Immunocytochemistry
Application Notes Immunofluorescence: A previous lot of this antibody was used in IF.

Western Blot: A previous lot of this antibody was used in WB.

Immunoprecipitation: A previous lot of this antibody was used in IP.

Immunohistochemistry: A previous lot of this antibody was used in IH.

Flow Cytometry: A starting range of 10-20 µg/mL is suggested.

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Human embryonal carcinoma cell line 2102Ep
Clone clone TRA-1-81
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity This antibody reacts with TRA-1-81 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. Both the TRA-1-81 and TRA-1-60 monoclonal antibodies (MAB4360) recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule.
Isotype IgM
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Gene Symbol
  • TRA-1-81
Purification Method Ammonium Sulfate Precipitation
Stem Cell Type
  • Human Embryonic Stem Cells
  • Induced Pluripotent Stem Cells
Molecular Weight 420/250 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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