Ms X Neurofilament 200 kD

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	IHC, IH(P), WB	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

IHC, IH(P), WB

Purified

Monoclonal Antibody

Description
Catalogue Number	MAB5262
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Neurofilament 200 kDa Antibody, clone RT97
Background Information	Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the Millipore Technical Support section.

Description

Catalogue Number

MAB5262

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Neurofilament 200 kDa Antibody, clone RT97

Background Information

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the Millipore Technical Support section.

Product Information
Format	Purified
Control	Brain Cultured neurons
Presentation	Purified mouse monoclonal IgG1 in 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide.

Product Information

Format

Purified

Control

Brain
Cultured neurons

Presentation

Purified mouse monoclonal IgG1 in 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide.

Applications
Application	Anti-Neurofilament 200 kDa Antibody, clone RT97 is an antibody against Neurofilament 200 kDa for use in IH, IH(P) & WB.
Key Applications	Immunohistochemistry Immunohistochemistry (Paraffin) Western Blotting
Application Notes	Immunohistochemistry: 1-10 μg/mL Immunohistochemistry(paraffin): Representative images from a previous lot. Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Human Cerebellum Optimal working dilutions must be determined by end user.

Applications

Application

Anti-Neurofilament 200 kDa Antibody, clone RT97 is an antibody against Neurofilament 200 kDa for use in IH, IH(P) & WB.

Key Applications

Immunohistochemistry
Immunohistochemistry (Paraffin)
Western Blotting

Application Notes

Immunohistochemistry:
1-10 μg/mL

Immunohistochemistry(paraffin):
Representative images from a previous lot.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Human Cerebellum

Optimal working dilutions must be determined by end user.

Biological Information
Immunogen	Rat brain protein preparation
Clone	RT97
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	The antibody recognizes an epitope present on human neurofilaments 200 kDa and both bovine 160 kDa and 200 kDa neurofilaments (2). lt reacts with numerous neurites in human senile plaques and decorates neurofibrillary tangles (NFT) (i.e. on the pyramidal cells of the hippocampus and on neurons of the cerebral cortex of subjects with senile dementia of the Alzheimer’s type).
Isotype	IgG1
Species Reactivity	Human Mouse Rat
Species Reactivity Note	Human and mouse. Expected to react with rat based on sequence homology.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_021076.2
Gene Symbol	NEFH NFH NF-H KIAA0845
Purification Method	Protein A Purfied
UniProt Number	P12036
UniProt Summary	FUNCTION: SwissProt: P12036 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. SIZE: 1026 amino acids; 112480 Da PTM: There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. & Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function. DISEASE: SwissProt: P12036 # Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors. SIMILARITY: SwissProt: P12036 ## Belongs to the intermediate filament family.
Molecular Weight	200 kDa

Biological Information

Immunogen

Rat brain protein preparation

Clone

RT97

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

The antibody recognizes an epitope present on human neurofilaments 200 kDa and both bovine 160 kDa and 200 kDa neurofilaments (2). lt reacts with numerous neurites in human senile plaques and decorates neurofibrillary tangles (NFT) (i.e. on the pyramidal cells of the hippocampus and on neurons of the cerebral cortex of subjects with senile dementia of the Alzheimer’s type).

Isotype

IgG1

Species Reactivity

Human
Mouse
Rat

Species Reactivity Note

Human and mouse. Expected to react with rat based on sequence homology.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_021076.2

Gene Symbol

NEFH
NFH
NF-H
KIAA0845

Purification Method

Protein A Purfied

UniProt Number

P12036

UniProt Summary

FUNCTION: SwissProt: P12036 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
SIZE: 1026 amino acids; 112480 Da
PTM: There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. & Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
DISEASE: SwissProt: P12036 # Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
SIMILARITY: SwissProt: P12036 ## Belongs to the intermediate filament family.

Molecular Weight

200 kDa

Product Usage Statements
Quality Assurance	Evaluated by Western Blot on Mouse brain membrane lysates. Western Blotting Analysis: 1:500 dilution of this antibody detected NEUROFILAMENT on 10 µg of Mouse brain membrane lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot on Mouse brain membrane lysates.

Western Blotting Analysis:
1:500 dilution of this antibody detected NEUROFILAMENT on 10 µg of Mouse brain membrane lysates.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8ºC from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8ºC from date of receipt.

Packaging Information
Material Size	50 µg

Packaging Information

Material Size

50 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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Ms X Neurofilament 200 kD

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