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Ms X SSEA-1

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货号：MAB4301

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, R FC, IF, IHC, IP M Purified Monoclonal Antibody
Description
Catalogue Number MAB4301
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480
Alternate Names
  • SSEA-1
  • CD15
  • Lewis X
  • 3-FAL
Background Information SSEA-1 is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.
References
Product Information
Format Purified
Control
  • P19 mouse embryonic carcinoma cells
Presentation Purified mouse monoclonal IgM in buffer containing 0.05M Potassium Phosphate, 0.3M NaCl, pH 8.0 with 0.05% Sodium Azide
Applications
Application Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480 detects level of Stage-Specific Embryonic Antigen-1 & has been published & validated for use in FC, IF, IH & IP.
Key Applications
  • Flow Cytometry
  • Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
Application Notes Immunohistochemistry:
A previous lot of this antibody was tested on IH.

Immunoprecipitation:
A previous lot of this antibody was tested on IP.

Immunofluorescence:
A previous lot of this antibody was tested on IF. 4% PFA fixed, 5-10 minutes room temperature. No detergent is required as epitope is external.

Flow Cytometry:
A starting range of 10-20 µg/mL is suggested.

Positive mouse EC lines include:
F9, PCC4, ND-1, SCC1, NG2, LT/SV, MH-15, FA-25.
Lines : PYS-2, OTT6050f, B3T3SV. C57SV, K129SV. KCA, QAIB, BW5147 were negative.

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen F9 tetracarcinoma stem cells (X-irradiated).
Clone MC-480
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity This antibody reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG).
Isotype IgM
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary FUT4 catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). It has been detected in human embryos (5-10 weeks) suggesting a role in development.
Gene Symbol
  • FUT4
  • ELFT
  • FCT3A
  • Fuc-TIV
  • 1,3fucosyltransferase
  • FUC-TIV
  • FucT-IV
  • CD15
  • Alpha-
  • EC 2.4.1.-
Purification Method Ammonium Sulfate Precipitation
UniProt Number
UniProt Summary FUNCTION: SwissProt: P22083 # May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.
SIZE: 405 amino acids; 45570 Da
SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi.
SIMILARITY: SwissProt: P22083 ## Belongs to the glycosyltransferase 10 family.
Stem Cell Type
  • Human Embryonic Stem Cells
  • Mouse Embryonic Stem Cells
  • Neural Stem Cells
  • Induced Pluripotent Stem Cells
Molecular Weight 220 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8ºC from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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