Rb X Rat Aquaporin 3

Species Reactivity	Key Applications	Host	Format	Antibody Type
M, H, R, Po, Ca	ELISA, ICC, IHC, WB	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

M, H, R, Po, Ca

ELISA, ICC, IHC, WB

Purified

Monoclonal Antibody

Description
Catalogue Number	MAB3067
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Connexin 43 Antibody, clone 4E6.2
Alternate Names	Gap junction 43 kDa heart protein connexin 43 gap junction protein, alpha 1, 43kDa gap junction protein, alpha 1, 43kDa (connexin 43) gap junction protein, alpha-like
Background Information	Connexins are highly homologous proteins that assemble as hexamers (connexons) to form gap junctions, small channels which allow for the passage of ions and other small molecules up to 1,200 daltons between neighboring cells. Gap junctions play an important role in a variety of cellular processes including homeostasis, intercellular metabolic cooperation and synchronization, morphogenesis, cell differentiation, and growth control. The most broadly studied connexins are the 26, 32, 43 and 45 kDa isoforms. Connexin 43 is a 382 a.a. protein belonging to the alpha-type (Group II) subfamily of connexin proteins and possessing four transmembrane regions, with cytoplasmic amino and carboxyl terminals. It undergoes rapid turnover in the cell and its monomers may reside in the ER/Golgi network, forming a reservoir available for assembly upon degradation of existing connexin-43 channels. Connexin 43 has been detected in a variety of tissues including: atrial and ventricular myocytes, granulose cells, astrocytes, fibroblasts, leptomeningeal cells, brain, lung, smooth muscle, uterine muscle, kidney, lens, epithelium, cornea and testis. Connexin 43 also may have a role in communication between migrating cardiac neural crest cells.

Description

Catalogue Number

MAB3067

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Connexin 43 Antibody, clone 4E6.2

Alternate Names

Gap junction 43 kDa heart protein
connexin 43
gap junction protein, alpha 1, 43kDa
gap junction protein, alpha 1, 43kDa (connexin 43)
gap junction protein, alpha-like

Background Information

Connexins are highly homologous proteins that assemble as hexamers (connexons) to form gap junctions, small channels which allow for the passage of ions and other small molecules up to 1,200 daltons between neighboring cells. Gap junctions play an important role in a variety of cellular processes including homeostasis, intercellular metabolic cooperation and synchronization, morphogenesis, cell differentiation, and growth control. The most broadly studied connexins are the 26, 32, 43 and 45 kDa isoforms. Connexin 43 is a 382 a.a. protein belonging to the alpha-type (Group II) subfamily of connexin proteins and possessing four transmembrane regions, with cytoplasmic amino and carboxyl terminals. It undergoes rapid turnover in the cell and its monomers may reside in the ER/Golgi network, forming a reservoir available for assembly upon degradation of existing connexin-43 channels. Connexin 43 has been detected in a variety of tissues including: atrial and ventricular myocytes, granulose cells, astrocytes, fibroblasts, leptomeningeal cells, brain, lung, smooth muscle, uterine muscle, kidney, lens, epithelium, cornea and testis. Connexin 43 also may have a role in communication between migrating cardiac neural crest cells.

Product Information
Format	Purified
Control	Mouse brain tissue lysate
Presentation	Purified mouse monoclonal IgG1 liquid in PBS containing sodium azide.

Product Information

Format

Purified

Control

Mouse brain tissue lysate

Presentation

Purified mouse monoclonal IgG1 liquid in PBS containing sodium azide.

Applications
Application	Anti-Connexin 43 Antibody, clone 4E6.2 detects level of Connexin 43 & has been published & validated for use in ELISA, IC, IH & WB.
Key Applications	ELISA Immunocytochemistry Immunohistochemistry Western Blotting
Application Notes	Western Blot (colorimetric detection): 1:1000-10,000 freshly prepared lysates, proteinase inhibitors are recommended. Recognizes proteins of molecular weight 43-47 kDa on Western blots of mouse brain lysate. Immunocytochemistry: A 1:50-1:250 dilution of a previous lot was used in immunocytochemistry. Immunohistochemistry: A previous lot was used to detect Connexin 43 expression in the keratinocytes of the middle and upper epidermal layers of frozen human skin tissue (Arita, Ken, et al. (2006). Am. J. Pathol. 169:416-423). Immunohistochemistry: A previous lot was used to detect Connexin 43 expression in human and rat heart tissue (1:500 dilution). ELISA: A previous lot of this antibody was used in ELISA. Optimal working dilutions must be determined by end user.

Applications

Application

Anti-Connexin 43 Antibody, clone 4E6.2 detects level of Connexin 43 & has been published & validated for use in ELISA, IC, IH & WB.

Key Applications

ELISA
Immunocytochemistry
Immunohistochemistry
Western Blotting

Application Notes

Western Blot (colorimetric detection):
1:1000-10,000 freshly prepared lysates, proteinase inhibitors are recommended. Recognizes proteins of molecular weight 43-47 kDa on Western blots of mouse brain lysate.

Immunocytochemistry:
A 1:50-1:250 dilution of a previous lot was used in immunocytochemistry.

Immunohistochemistry:
A previous lot was used to detect Connexin 43 expression in the keratinocytes of the middle and upper epidermal layers of frozen human skin tissue (Arita, Ken, et al. (2006). Am. J. Pathol. 169:416-423).

Immunohistochemistry:
A previous lot was used to detect Connexin 43 expression in human and rat heart tissue (1:500 dilution).

ELISA:
A previous lot of this antibody was used in ELISA.

Optimal working dilutions must be determined by end user.

Biological Information
Immunogen	A synthetic peptide corresponding to positions 252-270 of the mouse connexin 43 sequence. This sequence is identical in rat and bovine.
Epitope	C-terminus
Clone	4E6.2
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	Connexin-43. Recognizes proteins of molecular weight 43-47 kDa on Western blots of mouse brain lysate. Mouse connexin 43 sequence is identical in rat and bovine, and differs by one amino acid in pig. This sequence has no known homology with other connexins. Also effective for detection of Cx43 in mouse keratinocyte cells and on frozen sections of rat heart.
Isotype	IgG1
Species Reactivity	Mouse Human Rat Pig Canine
Species Reactivity Note	Expected to react with human based on sequence homology.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_000165.3
Entrez Gene Summary	This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia and heart malformations.
Gene Symbol	GJA1 CX43 GJAL Cx43 ODD DFNB38 ODDD ODOD SDTY3 Connexin-43
Purification Method	Protein A Purfied
UniProt Number	P17302
UniProt Summary	FUNCTION: SwissProt: P17302 # One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. SIZE: 382 amino acids; 43008 Da SUBUNIT: A connexon is composed of a hexamer of connexins. Interacts with SGSM3. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell junction, gap junction. TISSUE SPECIFICITY: Expressed in the heart and fetal cochlea. DISEASE: SwissProt: P17302 # Defects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type III and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances. & Defects in GJA1 may be the cause of syndactyly type III (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits, so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected. & Defects in GJA1 a the cause of hypoplastic left heart syndrome (HLHS) [MIM:241550]. HLHS refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis. SIMILARITY: SwissProt: P17302 ## Belongs to the connexin family. Alpha-type (group II) subfamily.
Molecular Weight	43-47 kDa

Biological Information

Immunogen

A synthetic peptide corresponding to positions 252-270 of the mouse connexin 43 sequence. This sequence is identical in rat and bovine.

Epitope

C-terminus

Clone

4E6.2

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

Connexin-43. Recognizes proteins of molecular weight 43-47 kDa on Western blots of mouse brain lysate. Mouse connexin 43 sequence is identical in rat and bovine, and differs by one amino acid in pig. This sequence has no known homology with other connexins. Also effective for detection of Cx43 in mouse keratinocyte cells and on frozen sections of rat heart.

Isotype

IgG1

Species Reactivity

Mouse
Human
Rat
Pig
Canine

Species Reactivity Note

Expected to react with human based on sequence homology.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_000165.3

Entrez Gene Summary

This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia and heart malformations.

Gene Symbol

GJA1
CX43
GJAL
Cx43
ODD
DFNB38
ODDD
ODOD
SDTY3
Connexin-43

Purification Method

Protein A Purfied

UniProt Number

P17302

UniProt Summary

FUNCTION: SwissProt: P17302 # One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.
SIZE: 382 amino acids; 43008 Da
SUBUNIT: A connexon is composed of a hexamer of connexins. Interacts with SGSM3.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.
TISSUE SPECIFICITY: Expressed in the heart and fetal cochlea.
DISEASE: SwissProt: P17302 # Defects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type III and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances. & Defects in GJA1 may be the cause of syndactyly type III (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits, so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected. & Defects in GJA1 a the cause of hypoplastic left heart syndrome (HLHS) [MIM:241550]. HLHS refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis.
SIMILARITY: SwissProt: P17302 ## Belongs to the connexin family. Alpha-type (group II) subfamily.

Molecular Weight

43-47 kDa

Product Usage Statements
Quality Assurance	Evaluated by Western Blot on rat brain lysates. Western Blot Analysis: 1:1000 dilution of this antibody detected Connexin 43 on 10 μg of rat brain lysates
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot on rat brain lysates.

Western Blot Analysis:
1:1000 dilution of this antibody detected Connexin 43 on 10 μg of rat brain lysates

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8ºC in undiluted aliquots from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8ºC in undiluted aliquots from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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