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Qiagen 凯杰 Biosharp Omega

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Rb X Somatostatin Receptor Type 1

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货号：AB9283

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
B, Ca, H, R, Sh, Fe IH(P) Rb Serum Polyclonal Antibody
Description
Catalogue Number AB928
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Follicle Stimulating Hormone Antibody
Alternate Names
  • FSH
  • LH
Background Information ANTIGEN:

Highly purified FSH
References
Product Information
Format Serum
Presentation Liquid antiserum. Contains 0.1% sodium azide.
Applications
Application Detect Follicle Stimulating Hormone using this Anti-Follicle Stimulating Hormone Antibody validated for use in IH(P).
Key Applications
  • Immunohistochemistry (Paraffin)
Application Notes Immunofluorescence and immunoperoxidase staining of formalin-fixed and paraffin-embedded sections of anterior pituitary glands. Cryostat sections of the same tissues can also be used.

Optimal working dilutions must be determined by the end user.

IMMUNOPEROXIDASE STAINING:

1. Hydrate the sections with PBS, pH 7.4.

2. Incubate the section with antiserum diluted 1:1000 in PBS at 37°C for 30 minutes.

3. Wash for 15 minutes with PBS at room temperature and treat sequentially with Goat anti-Rabbit IgG and PAP complex at 37°C. Wash excess reagent from the section as described above.

4. Treat the section with a mixture of 3,3'-diaminobenzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections may be dehydrated, cleared and mounted.

IMMUNOFLUORESCENCE STAINING:

1. Same as Step 1 above.

2. Incubate the section with antiserum diluted 1:20-1:40 in PBS at 37°C for 30 minutes.

3. After washing off the excess antiserum with PBS, treat the section with fluorescein labeled Goat anti-Rabbit IgG for 30 minutes at 37°C. Wash the tissue with PBS and mount under glycerine-saline.
Biological Information
Host Rabbit
Specificity Gives a positive and specific immunostaining of FSH-containing cells. Also shows reactivity to LH-containing cells.
Species Reactivity
  • Bovine
  • Canine
  • Human
  • Rat
  • Sheep
  • Feline
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary The pituitary glycoprotein hormone family includes follicle-stimulating hormone, luteinizing hormone, chorionic gonadotropin, and thyroid-stimulating hormone. All of these glycoproteins consist of an identical alpha subunit and a hormone-specific beta subunit. This gene encodes the beta subunit of follicle-stimulating hormone. In conjunction with luteinizing hormone, follicle-stimulating hormone induces egg and sperm production. Alternative splicing results in two transcript variants encoding the same protein.
Gene Symbol
  • FSHB
  • FSH-B
  • FSH-beta
  • follitropin
UniProt Number
UniProt Summary FUNCTION: SwissProt: P01225 # Stimulates development of follicle and spermatogenesis in the reproductive organs.
SIZE: 129 amino acids; 14700 Da
SUBUNIT: Heterodimer of a common alpha chain and a unique beta chain which confers biological specificity to thyrotropin, lutropin, follitropin and gonadotropin.
SUBCELLULAR LOCATION: Secreted.
DISEASE: SwissProt: P01225 # Defects in FSHB are a cause of isolated follicle- stimulating hormone deficiency (IFSHD) [MIM:229070]. Selective follicle-stimulating hormone deficiency is an uncommon cause of infertility, producing amenorrhea and hypogonadism in women and oligo or azoospermia with normal testosterone levels in normally virilised men.
SIMILARITY: SwissProt: P01225 ## Belongs to the glycoprotein hormones subunit beta family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at -20°C for up to 12 months in undiluted aliquots. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 500 µL
Transport Information
Supplemental Information
Specifications

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