Anti-逷ix, SH3 domain (rabbit polyclonal)

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Anti-逷ix, SH3 domain (rabbit polyclonal)

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货号：07-1450

品牌：Millipore 密理博

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	WB	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	07-1450-I
Replaces	07-1450
Description	Anti-Beta Pix, SH3 Domain Antibody
Alternate Names	Rho guanine nucleotide exchange factor 7 Beta-Pix COOL-1 PAK-interacting exchange factor beta p85
Background Information	Beta-Pix (ARHGEF7, βPix, COOL) is a Rho guanine nucleotide exchange factor (GEF). Beta-Pix is a p21-activated protein kinase (PAK)-interacting exchange factor (Pix, COOL, p85SPR) that has been identified as a putative guanine nucleotide exchange factor (GEF) for Rac/Cdc42. The variants of Beta-Pix isoforms exhibit different tissue distributions. Beta-Pix-a is expressed ubiquitously in all the tissues examined, and other isoforms are expressed mainly in the central nervous system. PAK, a Ser/Thr kinase, is an important mediator of Rho family activity interacts with Beta-Pix at the SH3 domain of Beta-Pix though its proline rich region. This interaction is thought to affect the GTP hydrolysis rate of Rac and Cdc42.

References

Product Information
Format	Affinity Purified
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	Detect Beta Pix using this Anti-Beta Pix antibody, SH3 Domain validated for use in western blotting.
Key Applications	Western Blotting
Application Notes	Western Blotting Analysis: 1 µg/mL from a representative lot detected Beta-Pix, SH3 Domain in 10 µg of PC-12 cell lysate.

Biological Information
Immunogen	GST-tagged recombinant protein corresponding to the SH3 domain of human Beta-Pix, SH3 Domain.
Epitope	SH3 domain
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Species Reactivity	Human Mouse Rat
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_001106983
Gene Symbol	ARHGEF7 COOL1 P85SPR PAK3BP PIXB
Purification Method	Affinity Purfied
UniProt Number	Q14155
Molecular Weight	~80 kDa observed

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in Jurkat cell lysate. Western Blotting Analysis: 0.2 µg/mL of this antibody detected Beta-Pix, SH3 Domain in 10 µg of Jurkat cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

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Anti-逷ix, SH3 domain (rabbit polyclonal)

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