ANTI-HA-RAS, PC 12-301

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	WB	Rb	Culture Supernatant	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

Culture Supernatant

Monoclonal Antibody

Description
Catalogue Number	04-775
Replaces	05-775
Description	Anti-Ha-Ras Antibody, clone MC57, rabbit monoclonal
Alternate Names	GTP- and GDP-binding peptide B GTPase HRas Ha-Ras1 proto-oncoprotein Ras family small GTP binding protein H-Ras Transforming protein p21 c-has/bas p21 protein c-ras-Ki-2 activated oncogene p19 H-RasIDX protein transformation gene: oncogene HAMSV v-Ha-ras Harvey rat sarcoma viral oncogene homolog
Background Information	Ras proteins are small GTP-binding proteins which, unlike the heterotrimeric G-proteins, contain all GTPase and effector functions within a single polypeptide. At least three isoforms of Ras exist, Ki-Ras, Ha-Ras and N-Ras, with distinct expression patterns but similar signaling activity. Ras is palmitoylated and farnesylated at the carboxy terminus, anchoring it in the membrane. In resting cells, Ras is loaded with GDP, and is activated subsequent to growth factor stimulation of receptors, which recruit Ras Guanine nucleotide Exchange Factors to the plane of the membrane. Proximity of exchange factors to the Ras proteins causes release of GDP, and its replacement by GTP. In its GTP-bound form, Ras binds several proteins, including Raf, RalGDS and PI3 Kinase. Inactivation of Ras occurs by GTP hydrolysis, which is greatly accelerated by RasGAP or NF-1, two known Ras GTPase Activating Proteins. It is possible to assay for Ras activation by incubation of lysates with the Ras-binding domain of Raf-1, which selectively binds to Ras:GTP.

Description

Catalogue Number

04-775

Replaces

05-775

Description

Anti-Ha-Ras Antibody, clone MC57, rabbit monoclonal

Alternate Names

GTP- and GDP-binding peptide B
GTPase HRas
Ha-Ras1 proto-oncoprotein
Ras family small GTP binding protein H-Ras
Transforming protein p21
c-has/bas p21 protein
c-ras-Ki-2 activated oncogene
p19 H-RasIDX protein
transformation gene: oncogene HAMSV
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Background Information

Ras proteins are small GTP-binding proteins which, unlike the heterotrimeric G-proteins, contain all GTPase and effector functions within a single polypeptide. At least three isoforms of Ras exist, Ki-Ras, Ha-Ras and N-Ras, with distinct expression patterns but similar signaling activity. Ras is palmitoylated and farnesylated at the carboxy terminus, anchoring it in the membrane. In resting cells, Ras is loaded with GDP, and is activated subsequent to growth factor stimulation of receptors, which recruit Ras Guanine nucleotide Exchange Factors to the plane of the membrane. Proximity of exchange factors to the Ras proteins causes release of GDP, and its replacement by GTP. In its GTP-bound form, Ras binds several proteins, including Raf, RalGDS and PI3 Kinase. Inactivation of Ras occurs by GTP hydrolysis, which is greatly accelerated by RasGAP or NF-1, two known Ras GTPase Activating Proteins. It is possible to assay for Ras activation by incubation of lysates with the Ras-binding domain of Raf-1, which selectively binds to Ras:GTP.

Product Information
Format	Culture Supernatant
Control	Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Presentation	Cultured supernantant containing 0.05% sodium azide.

Product Information

Format

Culture Supernatant

Control

Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Presentation

Cultured supernantant containing 0.05% sodium azide.

Applications
Application	Detect Ha-Ras using this Anti-Ha-Ras Antibody, clone MC57 validated for use in WB.
Key Applications	Western Blotting

Applications

Application

Detect Ha-Ras using this Anti-Ha-Ras Antibody, clone MC57 validated for use in WB.

Key Applications

Western Blotting

Biological Information
Immunogen	GST fusion protein corresponding to full length human Ha-Ras. Clone MC57.
Clone	MC57
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Recognizes Ha-Ras, Mr ~21 kDa. Does not cross react with Cdc42, Rho or Rac1.
Isotype	IgG
Species Reactivity	Human Mouse Rat
Species Reactivity Note	Human. Predicted to cross-react with mouse and rat based on sequence homology.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_005334.1
Entrez Gene Summary	This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus.Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene.
Gene Symbol	C-BAS/HAS C-H-RAS C-HA-RAS1 CTLO H-RASIDX H-Ras-1 HAMSV HRAS1 Ha-Ras K-RAS N-RAS OTTHUMP00000162769 OTTHUMP00000166053 OTTHUMP00000166055 RASH1 c-H-ras p21ras
UniProt Number	P01112
UniProt Summary	FUNCTION: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Ref.18 Ref.32 ENZYME REGULATION: Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP). SUBUNIT STRUCTURE: In its GTP-bound form interacts with PLCE1. Interacts with TBC1D10C. Interacts with RGL3 By similarity. Forms a signaling complex with RASGRP1 and DGKZ. Interacts with RASSF5. SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor. Note: Shuttles between the plasma membrane and the Golgi apparatus. PTM: Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi. S-nitrosylated; critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation. INVOLVEMENT IN DISEASE:Defects in HRAS are the cause of Costello syndrome [MIM:218040]; also known as faciocutaneoskeletal syndrome. Costello syndrome is a rare condition characterized by prenatally increased growth, postnatal growth deficiency, mental retardation, distinctive facial appearance, cardiovascular abnormalities (typically pulmonic stenosis, hypertrophic cardiomyopathy and/or atrial tachycardia), tumor predisposition, skin and musculoskeletal abnormalities. Defects in HRAS are the cause of congenital myopathy with excess of muscle spindles (CMEMS) [MIM:218040]. CMEMS is a variant of Costello syndrome. Defects in HRAS may be a cause of susceptibility to Hurthle cell thyroid carcinoma [MIM:607464]; also known as Hurthle cell thyroid neoplasia. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms. Mutations which change positions 12, 13 or 61 activate the potential of HRAS to transform cultured cells and are implicated in a variety of human tumors. Defects in HRAS are a cause of bladder cancer. Defects in HRAS are the cause of oral squamous cell carcinoma (OSCC). SEQUENCE SIMILARITIES: Belongs to the small GTPase superfamily. Ras family. MASS SPECTROMETRY: Molecular mass is 6.223±2 Da from positions 112 - 166. Determined by ESI. Molecular mass is 6.253±2 Da from positions 112 - 166. Determined by ESI. Includes one nitric oxide molecule.
Molecular Weight	~21 kDa

Biological Information

Immunogen

GST fusion protein corresponding to full length human Ha-Ras. Clone MC57.

Clone

MC57

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

Recognizes Ha-Ras, Mr ~21 kDa. Does not cross react with Cdc42, Rho or Rac1.

Isotype

IgG

Species Reactivity

Human
Mouse
Rat

Species Reactivity Note

Human. Predicted to cross-react with mouse and rat based on sequence homology.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_005334.1

Entrez Gene Summary

This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus.Mutations in this gene cause Costello syndrome, a disease
characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene.

Gene Symbol

C-BAS/HAS
C-H-RAS
C-HA-RAS1
CTLO
H-RASIDX
H-Ras-1
HAMSV
HRAS1
Ha-Ras
K-RAS
N-RAS
OTTHUMP00000162769
OTTHUMP00000166053
OTTHUMP00000166055
RASH1
c-H-ras
p21ras

UniProt Number

P01112

UniProt Summary

FUNCTION: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Ref.18 Ref.32
ENZYME REGULATION: Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).
SUBUNIT STRUCTURE: In its GTP-bound form interacts with PLCE1. Interacts with TBC1D10C. Interacts with RGL3 By similarity. Forms a signaling complex with RASGRP1 and DGKZ. Interacts with RASSF5.
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor. Note: Shuttles between the plasma membrane and the Golgi apparatus.
PTM: Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi. S-nitrosylated; critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation.
INVOLVEMENT IN DISEASE:Defects in HRAS are the cause of Costello syndrome [MIM:218040]; also known as faciocutaneoskeletal syndrome. Costello syndrome is a rare condition characterized by prenatally increased growth, postnatal growth deficiency, mental retardation, distinctive facial appearance, cardiovascular abnormalities (typically pulmonic stenosis, hypertrophic cardiomyopathy and/or atrial tachycardia), tumor predisposition, skin and musculoskeletal abnormalities.
Defects in HRAS are the cause of congenital myopathy with excess of muscle spindles (CMEMS) [MIM:218040]. CMEMS is a variant of Costello syndrome.
Defects in HRAS may be a cause of susceptibility to Hurthle cell thyroid carcinoma [MIM:607464]; also known as Hurthle cell thyroid neoplasia. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms. Mutations which change positions 12, 13 or 61 activate the potential of HRAS to transform cultured cells and are implicated in a variety of human tumors. Defects in HRAS are a cause of bladder cancer. Defects in HRAS are the cause of oral squamous cell carcinoma (OSCC).
SEQUENCE SIMILARITIES: Belongs to the small GTPase superfamily. Ras family.
MASS SPECTROMETRY: Molecular mass is 6.223±2 Da from positions 112 - 166. Determined by ESI. Molecular mass is 6.253±2 Da from positions 112 - 166. Determined by ESI. Includes one nitric oxide molecule.

Molecular Weight

~21 kDa

Product Usage Statements
Quality Assurance	Western Blot Analysis: A 1:1000 dilution of this antibody detected Ha-Ras in RIPA lysates from A431 cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Western Blot Analysis: A 1:1000 dilution of this antibody detected Ha-Ras in RIPA lysates from A431 cells.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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