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Rb X 14-3-3 pan

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货号：AB9748

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H IP, WB, IH(P) Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB9748-I
Replaces AB9748
Description Anti-14-3-3 Antibody (pan)
Alternate Names
  • 14-3-3 protein beta/alpha
  • KCIP-1
  • Protein 1054
  • Protein kinase C inhibitor protein 1
  • 14-3-3 protein epsilon
  • 14-3-3E
  • 14-3-3 protein eta
  • Protein AS1
  • 14-3-3 protein gamma
  • 14-3-3 protein theta
  • 14-3-3 protein T-cell
  • 14-3-3 protein tau
  • Protein HS1
  • 14-3-3 protein zeta/delta
Background Information 14-3-3 protein beta/alpha (KCIP-1, Protein 1054, Protein kinase C inhibitor protein 1), epsilon (14-3-3E), eta (Protein AS1), gamma (KCIP-1, Protein kinase C inhibitor protein 1), theta (14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1), zeta/delta (KCIP-1, Protein kinase C inhibitor protein 1) are encoded by the YWHAB, YWHAE, YWHAH, YWHAG, YWHAQ, YWHAZ gene (Gene ID 7529, 7531, 7533, 7532, 10971, 7534, respectively) in human. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers, thus rendering 14-3-3 as a key multifunctional regulatory molecule. 14-3-3 isomers have been implicated in several neurological disorders: for example isoforms gamma, epsilon and beta are expressed in the cerebrospinal fluid of Creutzfeldt-Jakob patients, but not patients suffering from other neurological conditions such as Alzheimer's. The 14-3-3 pan antibody is one of a panel of antibodies that were generated against 14-3-3 isoforms and have demonstrated their potential for use as an ELISA-based diagnostic tool. These antibodies will allow the research to investigate the role of this protein in key cellular processes and neurological disorders such as Creutzfeldt-Jakob, Alzheimer's and Parkinson's disease.
References
Product Information
Format Affinity Purified
Presentation Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application Anti-14-3-3 pan, Cat. No. AB9748-I, is a highly specific rabbit polyclonal antibody, that targets 14-3-3 protein and has been tested in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting and .
Key Applications
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application Notes Immunohistochemistry Analysis: A 1:5,000 dilution from a representative lot detected 14-3-3 immunoreactivity in human prostate cancer, cerebellum, and tonsil tissue sections.

Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated 14-3-3 from 500 µg of A431 cell lysate.
Biological Information
Immunogen A mixture of KLH-conjugated linear peptides corresponding the the N-terminal end sequences of human 14-3-3 alpha/beta (YWHAB), gamma (YWHAG), zeta/delta (YWHAZ), epsilon (YWHAE), eta (YWHAH or YWHA1), and theta (YWHAQ).
Concentration Please refer to lot specific datasheet.
Host Rabbit
Specificity This antibody targets the N-terminal end sequences of 14-3-3 alpha/beta (YWHAB), gamma (YWHAG), zeta/delta (YWHAZ), epsilon (YWHAE), eta (YWHAH or YWHA1), and theta (YWHAQ).
Species Reactivity
  • Human
Species Reactivity Note Human. Predicted to react with Chicken, Bovine, Fish, Porcine, Rabbit, Mouse, Rat, Sheep, Xenopus, Zebra Fish based on 100% sequence homology.
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • YWHAB
  • YWHAE
  • YWHAG
  • YWHAZ
  • YWHA1
  • YWHAH
  • YWHAQ
Purification Method Affinity purified.
UniProt Number
Molecular Weight ~28 kDa observed. 28.08/27.85 kDa (human isoform Long/Short; YWHAB), 27.75 kDa (human YWHAZ), 29.17 kDa (human YWHAE), 28.22 kDa (human YWHAH or YWHA1), 28.30 kDa (human YWHAG), 27.76 kDa (human YWHAQ) calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected 14-3-3 in 10 µg of A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 μg
Transport Information
Supplemental Information
Specifications

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