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Qiagen 凯杰 Biosharp Omega

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货号：AB15410

品牌：Millipore 密理博

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Replacement Information

特卖

100% Performance Guaranteed

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
B, Ca, H, R ICC, IHC, IH(P) Rb Serum Polyclonal Antibody
Description
Catalogue Number AB5804
Replaces AB1540
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Glial Fibrillary Acidic Protein (GFAP) Antibody
Alternate Names
  • glial fibrillary acidic protein
Background Information Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
References
Product Information
Format Serum
Control
  • Mouse brain tissue, rat astrocytes, rat neurons.
Presentation Rabbit serum. Liquid. Contains no preservative.
Applications
Application Anti-Glial Fibrillary Acidic Protein (GFAP) Antibody is an antibody against Glial Fibrillary Acidic Protein (GFAP) for use in IC, IH, IH(P) with more than 65 product citations.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunohistochemistry (Paraffin)
Application Notes Immunohistochemistry(paraffin):
Representative images from a previous lot.

Immunocytochemistry:
A 1:1,000 dilution of a previous lot was used in Immunocytochemistry.
Biological Information
Immunogen Purified bovine GFAP
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Recognizes Glial Fibrillary Acidic Protein (GFAP).
Species Reactivity
  • Bovine
  • Canine
  • Human
  • Rat
Species Reactivity Note Human, rat, bovine and canine. It is expected that the antibody will react with most mammalian GFAP due to homology. Reactivity with other species has not been confirmed.
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.
Gene Symbol
  • FLJ45472
  • GFAP
Purification Method Unpurified
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q28115 # GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
SIZE: 428 amino acids; 49512 Da
SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
SIMILARITY: SwissProt: Q28115 ## Belongs to the intermediate filament family.
Molecular Weight 50 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by immunohistochemistry on brain tissue, astrocytes, and neurons.

Immunohistochemistry(paraffin) Analysis:
GFAP (IHC2078) staining of Human Brain. Tissue pretreated with Citrate Buffer, pH 6.0. Pre-diluted polyclonal antibody, using IHC-Select® Detection with HRP-DAB. Glial cells stain strongly (brown).
Optimal Staining with Citrate Buffer Epitope Retrieval: Human Brain
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at -20°C undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size 50 µL
Transport Information
Supplemental Information
Specifications

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