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Qiagen 凯杰 Biosharp Omega

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UbcH7, active 250ug

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货号：23-047M

品牌：Millipore 密理博

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Replacement Information

重要规格表

Key Applications Species Cascade Component
Enzyme Assays Human E2
Description
Catalogue Number 23-047
Brand Family Upstate
Trade Name
  • Upstate
Description UbcH7 Protein, active, 10 µg
Alternate Names
  • Ubiquitin-conjugating enzyme E2 L3
  • L-UBC
  • Ubiquitin carrier protein L3
  • Ubiquitin-conjugating enzyme E2-F1
  • Ubiquitin-protein ligase L3
  • UBE2L3
  • UBCE7
Background Information Ubiquitin-conjugating (E2) enzymes are characterized by the presence of a highly conserved ubiquitin-conjugating domain, which accommodates ATP-activated ubiquitin (Ub) via a covalently linked thioester on its active-site residue. E2 enzymes act through selective protein-protein interactions with the ubiquitin-activating E1 enzyme and ubiquitin ligase E3 enzymes and are able to differentiate effects on downstream substrates, either with a single Ub molecule or a Ub chain. While E3s are involved in substrate selection, E2s are the main determinants for selection of the lysine to construct Ub chains, which thereby directly control the cellular fate of the substrate.

UbcH7 shows broad specificity for HECT-type E3s but does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine. Despite lacking this lysine reactivity, UbcH7 exhibits activity with the RBR family E3 enzymes, such as Parkin and ARIH1.
References
Product Information
Applications
Application Active, full length, recombinant human UbcH7. N-terminal HA, 6His-tagged. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.
Key Applications
  • Enzyme Assays
Biological Information
Cascade Component E2
Entrez Gene Number
Purification Method Purified using immobilized metal affinity chromatography.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size 10 µg
Transport Information
Supplemental Information
Specifications

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